CAJ | 학술논문

目的：观察三七总皂苷对脑出血大鼠脑组织含水量及凝血酶的影响，探讨其对脑出血的作用机制。方法 SD雄性大鼠随机分为空白组、假手术组、模型组、治疗组，分别观察6、24、48、72 h及7 d 5个时间点。采用尾壳核注入胶原酶肝素钠生理盐水溶液法建立大鼠脑出血模型，治疗组给予三七总皂苷腹腔注射，每日1次，采用干湿重法测量脑组织含水量，检测48 h纤维蛋白原含量(FIB)和凝血酶凝结时间(TT)。结果各时点模型组脑含水量与空白组、假手术组比较明显升高，差异有统计学意义(P＜0.05)；除6 h时点外，治疗组各时点与模型组和治疗组比较，脑含水量明显降低，差异有统计学意义(P＜0.05)。与模型组比较，治疗组 FIB 明显降低，差异有统计学意义(P＜0.05)；治疗组TT下降不明显，差异无统计学意义(P＞0.05)。结论三七总皂苷通过下调脑出血大鼠脑组织含水量、FIB含量及TT达到治疗脑出血的目的。
목적：관찰삼칠총조감대뇌출혈대서뇌조직함수량급응혈매적영향，탐토기대뇌출혈적작용궤제。방법 SD웅성대서수궤분위공백조、가수술조、모형조、치료조，분별관찰6、24、48、72 h급7 d 5개시간점。채용미각핵주입효원매간소납생리염수용액법건립대서뇌출혈모형，치료조급여삼칠총조감복강주사，매일1차，채용간습중법측량뇌조직함수량，검측48 h섬유단백원함량(FIB)화응혈매응결시간(TT)。결과각시점모형조뇌함수량여공백조、가수술조비교명현승고，차이유통계학의의(P＜0.05)；제6 h시점외，치료조각시점여모형조화치료조비교，뇌함수량명현강저，차이유통계학의의(P＜0.05)。여모형조비교，치료조 FIB 명현강저，차이유통계학의의(P＜0.05)；치료조TT하강불명현，차이무통계학의의(P＞0.05)。결론삼칠총조감통과하조뇌출혈대서뇌조직함수량、FIB함량급TT체도치료뇌출혈적목적。
Objective To investigate the effects of Panax notoginseng saponins (PNS) on the cerebral water content and thrombin in the rats with intracerebral hemorrhage (ICH), and explore its mechnism for treating ICH. Methods SD male rats were randomly divided into blank group, sham operation group, model group and treatment group, and time points of 6, 24, 48, 72 h and 7 d were observed. ICH rat model was induced by collagenase and heparin in saline solution injected into caudate nucleus. The treatment group was injected PNS intraperitoneally once a day, and the cerebral water content was assessed by dry-wet weight method. Both content of fibrinogen (FIB) and thrombin clotting time (TT) in 48 h were measured. Results The cerebral water content of model group increased at every time point compared with blank group and sham operation group (P<0.05). The cerebral water content of treatment group was significantly decreased at every time point except for 6 h compared with model group (P<0.05). FIB of treatment group decreased compared with model group (P<0.05), while TT of treatment group was not significantly decreased (P>0.05). Conclusion PNS may play a role in treating ICH by decreasing cerebral water content, FIB and TT of ICH rats.