中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
2期
42-45
,共4页
益气养阴方%急性髓细胞白血病%细胞凋亡%蛋白表达%小鼠
益氣養陰方%急性髓細胞白血病%細胞凋亡%蛋白錶達%小鼠
익기양음방%급성수세포백혈병%세포조망%단백표체%소서
Yiqi Yangyin Recipe%acute myeloid leukemia%apoptosis%protein expression%mice
目的：观察益气养阴方及其拆方对小鼠细胞凋亡率及相关蛋白c-myc、survivin的影响，探讨其作用机制。方法 NOD-SCID小鼠经60Co照射后，尾静脉注射KG1a细胞1×106个造模；随机分为对照组、全方组、扶正组、祛邪组。治疗组给予相应药物灌胃，对照组给予等体积生理盐水灌胃。采用流式细胞术检测细胞凋亡率，免疫组化法检测c-myc、survivin蛋白表达。结果与对照组比较，治疗组细胞凋亡率升高，差异有统计学意义(P＜0.01)；治疗组淋巴结c-myc、survivin蛋白表达降低，差异有统计学意义(P＜0.05)。全方组对各指标的影响均优于扶正组和祛邪组(P＜0.05)。结论益气养阴方通过降低 c-myc、survivin 蛋白表达，诱导白血病细胞凋亡，起到治疗作用。
目的：觀察益氣養陰方及其拆方對小鼠細胞凋亡率及相關蛋白c-myc、survivin的影響，探討其作用機製。方法 NOD-SCID小鼠經60Co照射後，尾靜脈註射KG1a細胞1×106箇造模；隨機分為對照組、全方組、扶正組、祛邪組。治療組給予相應藥物灌胃，對照組給予等體積生理鹽水灌胃。採用流式細胞術檢測細胞凋亡率，免疫組化法檢測c-myc、survivin蛋白錶達。結果與對照組比較，治療組細胞凋亡率升高，差異有統計學意義(P＜0.01)；治療組淋巴結c-myc、survivin蛋白錶達降低，差異有統計學意義(P＜0.05)。全方組對各指標的影響均優于扶正組和祛邪組(P＜0.05)。結論益氣養陰方通過降低 c-myc、survivin 蛋白錶達，誘導白血病細胞凋亡，起到治療作用。
목적：관찰익기양음방급기탁방대소서세포조망솔급상관단백c-myc、survivin적영향，탐토기작용궤제。방법 NOD-SCID소서경60Co조사후，미정맥주사KG1a세포1×106개조모；수궤분위대조조、전방조、부정조、거사조。치료조급여상응약물관위，대조조급여등체적생리염수관위。채용류식세포술검측세포조망솔，면역조화법검측c-myc、survivin단백표체。결과여대조조비교，치료조세포조망솔승고，차이유통계학의의(P＜0.01)；치료조림파결c-myc、survivin단백표체강저，차이유통계학의의(P＜0.05)。전방조대각지표적영향균우우부정조화거사조(P＜0.05)。결론익기양음방통과강저 c-myc、survivin 단백표체，유도백혈병세포조망，기도치료작용。
Objective To observe the effects of Yiqi Yangyin Recipe (supplementing qi and nourishing yin) and its different assembling on apoptosis rate and associated protein expression of c-myc and survivin in mice, and explore its mechanism. Methods NOD-SCID mice were injected with l×106 KG1a cells from the tail vein after irradiation of 60Co. Model mice were randomly divided into control group, whole recipe group, strengthening body resistance group, eliminating pathogens group. The treatment groups were given correspongding administration, and control group was given equal volume of NS. Flow cytometry was applied to measure apoptosis rate. The immunochemistry method was used to detect the expression of c-myc, survivin. Results Apoptosis rate in all treatment groups were increased (P<0.01). The expression of c-myc and survivin in lymph node tissues in all treatment groups were significantly declined compared with control group (P<0.05). The effect of whole recipe group on the all indices was superior to strengthening body resistance group and eliminating pathogens group (P<0.05). Conclusion Yiqi Yangyin Recipe can decrease the expressions of c-myc and survivin, induce apoptosis in leukemia cells, therefore shows the therapeutic effect.
