CAJ | 학술논문

目的：探讨Bcl-XL反义寡核苷酸（ Bcl-XL ASODN）对食管癌EC9706细胞和人食管癌裸鼠移植瘤组织的诱凋亡作用。方法：将合成的Bcl-XL ASODN通过阳离子脂质体介导转染EC9706细胞后，以吖啶橙荧光染色、流式细胞术和TUNEL技术检测ASODN组、细胞对照组、空白对照组和N-ODN组的凋亡率；用TUNEL技术检测ASODN组、N-ODN组和细胞对照组裸鼠移植瘤组织的细胞凋亡率。结果：吖啶橙荧光染色、流式细胞术和TUNEL检测结果显示，ASODN组、N-ODN组、空白对照组和细胞对照组的凋亡率比较，差异均有统计学意义（F＝26．853、38．537、28．541，P均＜0．001）；且ASODN组的凋亡率均高于细胞对照组、空白对照组和N-ODN组（P均＜0．05）；TUNEL法检测移植瘤组织的细胞凋亡率结果显示，细胞对照组、N-ODN组及ASODN组的凋亡率比较，差异有统计学意义（F＝56．375，P＜0．001）；且ASODN组高于细胞对照组及N-ODN组（P均＜0．05）。结论：Bcl-XL ASODN对食管癌EC9706细胞及食管癌裸鼠移植瘤组织均有诱凋亡作用。
목적：탐토Bcl-XL반의과핵감산（ Bcl-XL ASODN）대식관암EC9706세포화인식관암라서이식류조직적유조망작용。방법：장합성적Bcl-XL ASODN통과양리자지질체개도전염EC9706세포후，이아정등형광염색、류식세포술화TUNEL기술검측ASODN조、세포대조조、공백대조조화N-ODN조적조망솔；용TUNEL기술검측ASODN조、N-ODN조화세포대조조라서이식류조직적세포조망솔。결과：아정등형광염색、류식세포술화TUNEL검측결과현시，ASODN조、N-ODN조、공백대조조화세포대조조적조망솔비교，차이균유통계학의의（F＝26．853、38．537、28．541，P균＜0．001）；차ASODN조적조망솔균고우세포대조조、공백대조조화N-ODN조（P균＜0．05）；TUNEL법검측이식류조직적세포조망솔결과현시，세포대조조、N-ODN조급ASODN조적조망솔비교，차이유통계학의의（F＝56．375，P＜0．001）；차ASODN조고우세포대조조급N-ODN조（P균＜0．05）。결론：Bcl-XL ASODN대식관암EC9706세포급식관암라서이식류조직균유유조망작용。
Aim:To investigate the effects of inducing apoptosis on esophageal cancer EC 9706 cells and xenograft tis-sue of human esophageal cancer in nude mice treated by Bcl-XL antisense oligodeoxynucleotide ( ASODN) .Methods:Bcl-XL ASODN was transfected into EC9706 cells following cationic liposome(ASODN group).N-ODN group, the cell control group and the blank control group were the control .The apoptosis rate of each cell groups was detected by acridine orange ( AO) fluorescent staining , flow cytometry and TUNEL , respectively .The apoptosis rate in xenograft tissue from nude mice in ASODN group, N-ODN group and the cell control group was detected by TUNEL .Resul ts:The results of AO fluores-cent staining, flow cytometry and TUNEL showed that there were significant differences in apoptosis rates among ASODN group,the cell control group, the blank control group and N-ODN group(F=26.853,38.537, 28.541,P<0.001).The ap-optosis rate was higher in ASODN group compared with the cell control ,the blank control and NO-DN groups(P<0.05). The apoptosis rates in xenograft tissue detected by TUNEL showed that there were significant differences among ASODN group, the cell control group and N-ODN group(F =563.75, P<0.001).The apoptosis rate was higher in ASODN group compared with the cell control group and N-ODN group(P<0.05).Conclusion:Bcl-XL ASODN could effectively induce apoptosis of EC9706 cell and xenograft tissue of human esophageal carcinoma .