CAJ | 학술논문

目的：探讨骨髓单个核细胞（BMMNCs）移植对脑出血（ICH）大鼠的影响。方法：通过密度梯度离心法提取大鼠BMMNCs并经流式细胞仪鉴定；采用立体定向技术向尾状核内注射Ⅶ型胶原酶构建大鼠ICH模型；24只大鼠分为PBS组和BMMNCs组，每组12只，于移植后第1、3、7和14天进行神经功能缺损评分（ mNSS）。90只大鼠分为假手术组、PBS组和BMMNCS组，每组30只，分别于移植后第0、1、3、7和14天处死6只大鼠检测脑组织含水量；移植后第7天行甲酚紫染色观察大脑皮层神经元数量；移植后第28天在荧光显微镜下观察BMMNCs在脑内的存活、分布和分化情况。结果：BMMNCs移植后不同时间点，与PBS组相比，大鼠神经功能恢复较好（ F时间＝15．243， F组间＝22．826， F交互＝4．932， P 均＜0．05），大鼠脑组织含水量明显降低（ F ＝27．518、106．965、45．006， P均＜0．001），大脑皮层神经元数量增多。 BMMNCs能够迁移分布至大鼠脑损伤区，并分化为神经元和星形胶质细胞。结论：静脉移植BMMNCs可减轻ICH大鼠脑水肿，BMMNCs能够在出血周边存活并分化为神经元和星形胶质细胞，改善ICH大鼠神经功能。
목적：탐토골수단개핵세포（BMMNCs）이식대뇌출혈（ICH）대서적영향。방법：통과밀도제도리심법제취대서BMMNCs병경류식세포의감정；채용입체정향기술향미상핵내주사Ⅶ형효원매구건대서ICH모형；24지대서분위PBS조화BMMNCs조，매조12지，우이식후제1、3、7화14천진행신경공능결손평분（ mNSS）。90지대서분위가수술조、PBS조화BMMNCS조，매조30지，분별우이식후제0、1、3、7화14천처사6지대서검측뇌조직함수량；이식후제7천행갑분자염색관찰대뇌피층신경원수량；이식후제28천재형광현미경하관찰BMMNCs재뇌내적존활、분포화분화정황。결과：BMMNCs이식후불동시간점，여PBS조상비，대서신경공능회복교호（ F시간＝15．243， F조간＝22．826， F교호＝4．932， P 균＜0．05），대서뇌조직함수량명현강저（ F ＝27．518、106．965、45．006， P균＜0．001），대뇌피층신경원수량증다。 BMMNCs능구천이분포지대서뇌손상구，병분화위신경원화성형효질세포。결론：정맥이식BMMNCs가감경ICH대서뇌수종，BMMNCs능구재출혈주변존활병분화위신경원화성형효질세포，개선ICH대서신경공능。
Aim:To investigate the effect of transplanted bone marrow mononuclear cells ( BMMNCs ) in a rat model of intracerebral hemorrhage ( ICH) .Methods:BMMNCs were isolated by Ficoll density gradient centrifugation .Cell sur-face antigens of CD34, CD45 and CD90 were identified by flow cytometry .Intracerebral hemorrhage models were estab-lished by stereotactic injection of collagenase Ⅶinto caudate putamen .Rats were randomly divided into sham group , PBS group and BMMNCs group .Functional deficits were assessed with the modified neurological severity score on the 1st, 3rd, 7th, and 14th day after transplantation.The number of neurons around hematoma was observed by Niss's staining on day 7 after transplantation.The survival, migration, distribution and differentiation of BMMNCs were assessed by immunofluores-cence double staining on day 28 after transplantation .Results:At different times after transplantation ,BMMNC transplan-tation significantly improved neurological function (Ftime =15.243, Fgroup =22.826,Finteraction =4.932,P<0.05), reduced brain edema(F =27.518,106.965,45.006,P <0.001), and increased the number of neurons compared with other groups.BMMNCs were able to migrate into perihematoma region and differentiated into neurons and astrocytes .Conclu-sion:BMMNCs transplantation can significantly reduce brain edema and promote neurological recovery by differentiating in -to neurons and astrocytes in a rat model of ICH .