郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
2期
247-250
,共4页
姚俊阁%李红雨%赵书君%张慧%张艳艳%郭欢欢
姚俊閣%李紅雨%趙書君%張慧%張豔豔%郭歡歡
요준각%리홍우%조서군%장혜%장염염%곽환환
SOX2%CyclinD1%卵巢上皮性癌
SOX2%CyclinD1%卵巢上皮性癌
SOX2%CyclinD1%란소상피성암
SOX2%CyclinD1%epithelial ovarian cancer
目的:检测卵巢上皮性癌组织中SOX2和CyclinD1的表达。方法:分别采用免疫组化SP法和RT-PCR法检测20例正常卵巢上皮组织、20例卵巢良性上皮性肿瘤组织及43例卵巢上皮性癌组织中SOX2和CyclinD1蛋白及mRNA的表达。结果:3种组织间SOX2、CyclinD1蛋白及mRNA的表达差异有统计学意义(蛋白:χ2=32.998,mRNA:F=454.222、398.284,P均<0.05);卵巢上皮性癌组织中SOX2、CyclinD1蛋白及mRNA的表达高于卵巢良性上皮性肿瘤组织及正常卵巢上皮组织( P均<0.05)。 SOX2、CyclinD1蛋白的表达均与卵巢上皮性癌FIGO分期、分化程度及淋巴结转移有关(P均<0.05)。卵巢上皮性癌组织中SOX2、CyclinD1蛋白的表达有关联(rp =0.422,P<0.05)。结论:SOX2和CyclinD1在卵巢上皮性癌组织中高表达,二者可能协同参与卵巢上皮性癌的发生及发展。
目的:檢測卵巢上皮性癌組織中SOX2和CyclinD1的錶達。方法:分彆採用免疫組化SP法和RT-PCR法檢測20例正常卵巢上皮組織、20例卵巢良性上皮性腫瘤組織及43例卵巢上皮性癌組織中SOX2和CyclinD1蛋白及mRNA的錶達。結果:3種組織間SOX2、CyclinD1蛋白及mRNA的錶達差異有統計學意義(蛋白:χ2=32.998,mRNA:F=454.222、398.284,P均<0.05);卵巢上皮性癌組織中SOX2、CyclinD1蛋白及mRNA的錶達高于卵巢良性上皮性腫瘤組織及正常卵巢上皮組織( P均<0.05)。 SOX2、CyclinD1蛋白的錶達均與卵巢上皮性癌FIGO分期、分化程度及淋巴結轉移有關(P均<0.05)。卵巢上皮性癌組織中SOX2、CyclinD1蛋白的錶達有關聯(rp =0.422,P<0.05)。結論:SOX2和CyclinD1在卵巢上皮性癌組織中高錶達,二者可能協同參與卵巢上皮性癌的髮生及髮展。
목적:검측란소상피성암조직중SOX2화CyclinD1적표체。방법:분별채용면역조화SP법화RT-PCR법검측20례정상란소상피조직、20례란소량성상피성종류조직급43례란소상피성암조직중SOX2화CyclinD1단백급mRNA적표체。결과:3충조직간SOX2、CyclinD1단백급mRNA적표체차이유통계학의의(단백:χ2=32.998,mRNA:F=454.222、398.284,P균<0.05);란소상피성암조직중SOX2、CyclinD1단백급mRNA적표체고우란소량성상피성종류조직급정상란소상피조직( P균<0.05)。 SOX2、CyclinD1단백적표체균여란소상피성암FIGO분기、분화정도급림파결전이유관(P균<0.05)。란소상피성암조직중SOX2、CyclinD1단백적표체유관련(rp =0.422,P<0.05)。결론:SOX2화CyclinD1재란소상피성암조직중고표체,이자가능협동삼여란소상피성암적발생급발전。
Aim:To explore the expressions and clinical significance of SOX 2 and CyclinD1 in epithelial ovarian canc-er tissue.Methods:Immunohistochemical SP method and RT-PCR were used to separately detect the expressions of SOX 2 and CyclinD1 in 20 cases of normal epithelial ovarian tissue , 20 cases of benign epithelial ovarian neoplasm tissue , and 43 cases of epithelial ovarian cancer tissue .Results:The expressions of SOX2 and CyclinD1 protein and mRNA in epithelial ovarian cancer tissue were significantly higher than those in benign epithelial ovarian neoplasm tissue and normal epithelial ovarian tissue(protein:χ2 =32.998;mRNA:F=454.222,398.284, all P<0.05).The expressions of SOX2 and CyclinD1 protein in epithelial ovarian cancer tissue were related to FIGO stage , histological differentiation and lymph node metastasis (P<0.05).The expression of SOX2 protein was associated with that of CyclinD1 protein in epithelial ovarian cancer tissue (rp =0.422,P<0.05).Conclusion: SOX2 and CyclinD1 are both over-expressed in epithelial ovarian cancer tissue . SOX2 may be in coordinated with CyclinD 1 in carcinogenesis and progression of epithelial ovarian cancer .