CAJ | 학술논문

目的：检测卵巢上皮性癌组织中SOX2和CyclinD1的表达。方法：分别采用免疫组化SP法和RT-PCR法检测20例正常卵巢上皮组织、20例卵巢良性上皮性肿瘤组织及43例卵巢上皮性癌组织中SOX2和CyclinD1蛋白及mRNA的表达。结果：3种组织间SOX2、CyclinD1蛋白及mRNA的表达差异有统计学意义（蛋白：χ2＝32．998，mRNA：F＝454．222、398．284，P均＜0．05）；卵巢上皮性癌组织中SOX2、CyclinD1蛋白及mRNA的表达高于卵巢良性上皮性肿瘤组织及正常卵巢上皮组织（ P均＜0．05）。 SOX2、CyclinD1蛋白的表达均与卵巢上皮性癌FIGO分期、分化程度及淋巴结转移有关（P均＜0．05）。卵巢上皮性癌组织中SOX2、CyclinD1蛋白的表达有关联（rp ＝0．422，P＜0．05）。结论：SOX2和CyclinD1在卵巢上皮性癌组织中高表达，二者可能协同参与卵巢上皮性癌的发生及发展。
목적：검측란소상피성암조직중SOX2화CyclinD1적표체。방법：분별채용면역조화SP법화RT-PCR법검측20례정상란소상피조직、20례란소량성상피성종류조직급43례란소상피성암조직중SOX2화CyclinD1단백급mRNA적표체。결과：3충조직간SOX2、CyclinD1단백급mRNA적표체차이유통계학의의（단백：χ2＝32．998，mRNA：F＝454．222、398．284，P균＜0．05）；란소상피성암조직중SOX2、CyclinD1단백급mRNA적표체고우란소량성상피성종류조직급정상란소상피조직（ P균＜0．05）。 SOX2、CyclinD1단백적표체균여란소상피성암FIGO분기、분화정도급림파결전이유관（P균＜0．05）。란소상피성암조직중SOX2、CyclinD1단백적표체유관련（rp ＝0．422，P＜0．05）。결론：SOX2화CyclinD1재란소상피성암조직중고표체，이자가능협동삼여란소상피성암적발생급발전。
Aim:To explore the expressions and clinical significance of SOX 2 and CyclinD1 in epithelial ovarian canc-er tissue.Methods:Immunohistochemical SP method and RT-PCR were used to separately detect the expressions of SOX 2 and CyclinD1 in 20 cases of normal epithelial ovarian tissue , 20 cases of benign epithelial ovarian neoplasm tissue , and 43 cases of epithelial ovarian cancer tissue .Results:The expressions of SOX2 and CyclinD1 protein and mRNA in epithelial ovarian cancer tissue were significantly higher than those in benign epithelial ovarian neoplasm tissue and normal epithelial ovarian tissue(protein:χ2 =32.998;mRNA:F=454.222,398.284, all P<0.05).The expressions of SOX2 and CyclinD1 protein in epithelial ovarian cancer tissue were related to FIGO stage , histological differentiation and lymph node metastasis (P<0.05).The expression of SOX2 protein was associated with that of CyclinD1 protein in epithelial ovarian cancer tissue (rp =0.422,P<0.05).Conclusion: SOX2 and CyclinD1 are both over-expressed in epithelial ovarian cancer tissue . SOX2 may be in coordinated with CyclinD 1 in carcinogenesis and progression of epithelial ovarian cancer .