郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
2期
243-246
,共4页
单峤%王新军%王世勋%李培栋%吴建珩%李晓辉
單嶠%王新軍%王世勛%李培棟%吳建珩%李曉輝
단교%왕신군%왕세훈%리배동%오건형%리효휘
X连锁凋亡抑制蛋白%人脑胶质瘤%半胱氨酸蛋白酶
X連鎖凋亡抑製蛋白%人腦膠質瘤%半胱氨痠蛋白酶
X련쇄조망억제단백%인뇌효질류%반광안산단백매
X-linked inhibitor of apoptosis protein%human glioma%caspase
目的：探讨X连锁凋亡抑制蛋白（ XIAP）在人脑胶质瘤中的表达情况。方法：收集低级别胶质瘤31例、高级别胶质瘤36例、正常脑组织35例，分别采用RT-PCR、Western blot 和免疫组织化学染色方法测定脑组织中XIAP mRNA及蛋白表达水平。结果：低、高级别胶质瘤组织中XIAP mRNA及蛋白表达水平较正常脑组织显著上调，高级别胶质瘤组织中XIAP mRNA及蛋白表达水平较低级别胶质瘤组织显著上调（F＝118．659和127．312，P＜0．001）。结论：XIAP在人脑胶质瘤中呈高表达，且与胶质瘤的病理分级有关，可能参与人脑胶质瘤的发生和进展。
目的：探討X連鎖凋亡抑製蛋白（ XIAP）在人腦膠質瘤中的錶達情況。方法：收集低級彆膠質瘤31例、高級彆膠質瘤36例、正常腦組織35例，分彆採用RT-PCR、Western blot 和免疫組織化學染色方法測定腦組織中XIAP mRNA及蛋白錶達水平。結果：低、高級彆膠質瘤組織中XIAP mRNA及蛋白錶達水平較正常腦組織顯著上調，高級彆膠質瘤組織中XIAP mRNA及蛋白錶達水平較低級彆膠質瘤組織顯著上調（F＝118．659和127．312，P＜0．001）。結論：XIAP在人腦膠質瘤中呈高錶達，且與膠質瘤的病理分級有關，可能參與人腦膠質瘤的髮生和進展。
목적：탐토X련쇄조망억제단백（ XIAP）재인뇌효질류중적표체정황。방법：수집저급별효질류31례、고급별효질류36례、정상뇌조직35례，분별채용RT-PCR、Western blot 화면역조직화학염색방법측정뇌조직중XIAP mRNA급단백표체수평。결과：저、고급별효질류조직중XIAP mRNA급단백표체수평교정상뇌조직현저상조，고급별효질류조직중XIAP mRNA급단백표체수평교저급별효질류조직현저상조（F＝118．659화127．312，P＜0．001）。결론：XIAP재인뇌효질류중정고표체，차여효질류적병리분급유관，가능삼여인뇌효질류적발생화진전。
Aim:To investigate the expression and significance of X-linked inhibitor of apoptosis protein ( XIAP) in human glioma .Methods:There were 31 cases of low grade glioma ,36 cases of high grade glioma and 35 cases normal brain tissue.The expressions of XIAP mRNA and protein were detected by RT-PCR,Western blot and immunohistochemis-try.Results:The expression levels of XIAP mRNA and protein were significantly higher in human glioma tissue than those in normal brain tissue ,and the expression levels of XIAP mRNA and protein were significantly higher in high -grade glioma group than those in low-grade glioma group(F=118.659 and 127.312,P<0.001).Conclusion:The mRNA and protein expression levels of XIAP were significantly high in human glioma which are associated with the grade of glioma .Therefore, XIAP may participate in the development and progression of human glioma .
