CAJ | 학술논문

目的：探讨局部晚期宫颈癌（ LACC）组织中ERCC1 mRNA表达和DNA倍体与新辅助化疗（ NACT）敏感性的关系。方法：收集60例LACC患者NACT前宫颈活检标本，应用PCR结合实时荧光探针技术分析标本中ER-CC1 mRNA的表达情况，采用细胞DNA定量分析技术分析DNA倍体。结果：60例患者中NACT有效33例，有效率为55．0％。 LACC组织中ERCC1 mRNA表达水平、DNA倍体与NACT疗效有关（ P＜0．05）；logistic回归模型分析结果也显示，LACC组织中ERCC1 mRNA高表达是NACT疗效的负性影响因素[β＝-2．672，OR（95％CI）＝0．069（0．008～0．583）]， DNA二倍体则是正性影响因素[β＝1．348，OR（95％CI）＝3．850（1．134～13．075）]。结论：LACC组织中ERCC1、DNA倍体表达与NACT敏感性密切相关。
목적：탐토국부만기궁경암（ LACC）조직중ERCC1 mRNA표체화DNA배체여신보조화료（ NACT）민감성적관계。방법：수집60례LACC환자NACT전궁경활검표본，응용PCR결합실시형광탐침기술분석표본중ER-CC1 mRNA적표체정황，채용세포DNA정량분석기술분석DNA배체。결과：60례환자중NACT유효33례，유효솔위55．0％。 LACC조직중ERCC1 mRNA표체수평、DNA배체여NACT료효유관（ P＜0．05）；logistic회귀모형분석결과야현시，LACC조직중ERCC1 mRNA고표체시NACT료효적부성영향인소[β＝-2．672，OR（95％CI）＝0．069（0．008～0．583）]， DNA이배체칙시정성영향인소[β＝1．348，OR（95％CI）＝3．850（1．134～13．075）]。결론：LACC조직중ERCC1、DNA배체표체여NACT민감성밀절상관。
Aim:To explore relationship between the effect of neoadjuvant chemotherapy ( NACT) and the expression of ERCC1 mRNA,DNA ploidy in locally advanced cervical cancer ( LACC) tissue.Methods:A total of 60 cases of LACC biopsy specimens before chemotherapy were collected .PCR combined with the fluorescence probe technique were applied to analyze the expression of ERCC 1 mRNA in the specimens , and the DNA quantitative analysis technique was adopted to an-alyze DNA ploidy.Results:Thirty-three cases were effective in the 60 patients, and the effective rate was 55.0%.There was a correlation between ERCC1 mRNA expression, DNA ploidy and the curative effect of NACT(P<0.05).The result of logistic regression showed that high expression of ERCC 1 mRNA was a negative factor for curative effect of NACT [β=-2.672,OR(95%CI) =0.069 (0.008 -0.583)],and DNA diploid was a positive factor [β=1.348,OR(95%CI)=3.850(1.134-13.075)].Conclusion:The expression of ERCC1 mRNA and DNA ploidy in LACC tissue may be related to the sensitivity of NACT .