郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
2期
233-236
,共4页
马一鸣%陈红敏%邓君丽%阎夏%窦萌萌%王莉
馬一鳴%陳紅敏%鄧君麗%閻夏%竇萌萌%王莉
마일명%진홍민%산군려%염하%두맹맹%왕리
局部晚期宫颈癌%ERCC1%DNA倍体%新辅助化疗
跼部晚期宮頸癌%ERCC1%DNA倍體%新輔助化療
국부만기궁경암%ERCC1%DNA배체%신보조화료
locally advanced cervical cancer%ERCC1%DNA ploidy%neoadjuvant chemotherapy
目的:探讨局部晚期宫颈癌( LACC)组织中ERCC1 mRNA表达和DNA倍体与新辅助化疗( NACT)敏感性的关系。方法:收集60例LACC患者NACT前宫颈活检标本,应用PCR结合实时荧光探针技术分析标本中ER-CC1 mRNA的表达情况,采用细胞DNA定量分析技术分析DNA倍体。结果:60例患者中NACT有效33例,有效率为55.0%。 LACC组织中ERCC1 mRNA表达水平、DNA倍体与NACT疗效有关( P<0.05);logistic回归模型分析结果也显示,LACC组织中ERCC1 mRNA高表达是NACT疗效的负性影响因素[β=-2.672,OR(95%CI)=0.069(0.008~0.583)], DNA二倍体则是正性影响因素[β=1.348,OR(95%CI)=3.850(1.134~13.075)]。结论:LACC组织中ERCC1、DNA倍体表达与NACT敏感性密切相关。
目的:探討跼部晚期宮頸癌( LACC)組織中ERCC1 mRNA錶達和DNA倍體與新輔助化療( NACT)敏感性的關繫。方法:收集60例LACC患者NACT前宮頸活檢標本,應用PCR結閤實時熒光探針技術分析標本中ER-CC1 mRNA的錶達情況,採用細胞DNA定量分析技術分析DNA倍體。結果:60例患者中NACT有效33例,有效率為55.0%。 LACC組織中ERCC1 mRNA錶達水平、DNA倍體與NACT療效有關( P<0.05);logistic迴歸模型分析結果也顯示,LACC組織中ERCC1 mRNA高錶達是NACT療效的負性影響因素[β=-2.672,OR(95%CI)=0.069(0.008~0.583)], DNA二倍體則是正性影響因素[β=1.348,OR(95%CI)=3.850(1.134~13.075)]。結論:LACC組織中ERCC1、DNA倍體錶達與NACT敏感性密切相關。
목적:탐토국부만기궁경암( LACC)조직중ERCC1 mRNA표체화DNA배체여신보조화료( NACT)민감성적관계。방법:수집60례LACC환자NACT전궁경활검표본,응용PCR결합실시형광탐침기술분석표본중ER-CC1 mRNA적표체정황,채용세포DNA정량분석기술분석DNA배체。결과:60례환자중NACT유효33례,유효솔위55.0%。 LACC조직중ERCC1 mRNA표체수평、DNA배체여NACT료효유관( P<0.05);logistic회귀모형분석결과야현시,LACC조직중ERCC1 mRNA고표체시NACT료효적부성영향인소[β=-2.672,OR(95%CI)=0.069(0.008~0.583)], DNA이배체칙시정성영향인소[β=1.348,OR(95%CI)=3.850(1.134~13.075)]。결론:LACC조직중ERCC1、DNA배체표체여NACT민감성밀절상관。
Aim:To explore relationship between the effect of neoadjuvant chemotherapy ( NACT) and the expression of ERCC1 mRNA,DNA ploidy in locally advanced cervical cancer ( LACC) tissue.Methods:A total of 60 cases of LACC biopsy specimens before chemotherapy were collected .PCR combined with the fluorescence probe technique were applied to analyze the expression of ERCC 1 mRNA in the specimens , and the DNA quantitative analysis technique was adopted to an-alyze DNA ploidy.Results:Thirty-three cases were effective in the 60 patients, and the effective rate was 55.0%.There was a correlation between ERCC1 mRNA expression, DNA ploidy and the curative effect of NACT(P<0.05).The result of logistic regression showed that high expression of ERCC 1 mRNA was a negative factor for curative effect of NACT [β=-2.672,OR(95%CI) =0.069 (0.008 -0.583)],and DNA diploid was a positive factor [β=1.348,OR(95%CI)=3.850(1.134-13.075)].Conclusion:The expression of ERCC1 mRNA and DNA ploidy in LACC tissue may be related to the sensitivity of NACT .