郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
2期
230-233
,共4页
郭燕%张东铭%刘爱萍%付艳芹%张苏河
郭燕%張東銘%劉愛萍%付豔芹%張囌河
곽연%장동명%류애평%부염근%장소하
妊娠%高脂高糖饮食%胰岛素抵抗%Tribbles同源蛋白3%Akt%饮食干预%大鼠
妊娠%高脂高糖飲食%胰島素牴抗%Tribbles同源蛋白3%Akt%飲食榦預%大鼠
임신%고지고당음식%이도소저항%Tribbles동원단백3%Akt%음식간예%대서
gestation%high sucrose and fat diet%insulin resistence%tribbles homologous protein 3%Akt%dietary inter-vention%rat
目的:观察饮食干预对高脂高糖妊娠大鼠肝组织中 Tribbles同源蛋白3(TRB3)和Akt mRNA的表达及胰岛素抵抗的影响。方法:75只健康雌性SD大鼠随机分为高脂高糖妊娠组、高脂高糖非妊娠组、普通饮食妊娠组、普通饮食非妊娠组及高脂高糖饮食妊娠后普通饮食干预组(干预组)5组,每组15只。妊娠第20天,用全自动生化检测仪检测FBG,ELISA试剂盒测定FINS,由此计算HOMA-IR。应用RT-PCR测定大鼠肝组织中TRB3和Akt mRNA的表达水平。结果:妊娠、高脂高糖饮食均可致大鼠HOMA-IR升高( F妊娠=2318.491,F饮食=2888.237,F交互=993.094, P均<0.001),同时大鼠肝组织中TRB3 mRNA的表达水平升高(F妊娠=256.887,F饮食=1749.406,F交互=2.579, P均<0.001),Akt mRNA的表达水平降低(F妊娠=221.091,F饮食=1416.984,F交互=28.918,P均<0.001)。高脂高糖妊娠组、干预组和普通饮食妊娠组HOMA-IR、TRB3和Akt mRNA的表达水平差异均有统计学意义( F=2772.745、445.896和390.334,P均<0.001);干预组HOMA-IR、TRB3 mRNA的表达水平较高脂高糖妊娠组降低,但仍高于普通饮食妊娠组,Akt mRNA的表达水平较高脂高糖妊娠组升高,但仍低于普通饮食妊娠组(P<0.05)。结论:TRB3和Akt可能参与了妊娠期胰岛素抵抗的发生发展,饮食干预可改善TRB3/Akt通路,减轻妊娠期胰岛素抵抗。
目的:觀察飲食榦預對高脂高糖妊娠大鼠肝組織中 Tribbles同源蛋白3(TRB3)和Akt mRNA的錶達及胰島素牴抗的影響。方法:75隻健康雌性SD大鼠隨機分為高脂高糖妊娠組、高脂高糖非妊娠組、普通飲食妊娠組、普通飲食非妊娠組及高脂高糖飲食妊娠後普通飲食榦預組(榦預組)5組,每組15隻。妊娠第20天,用全自動生化檢測儀檢測FBG,ELISA試劑盒測定FINS,由此計算HOMA-IR。應用RT-PCR測定大鼠肝組織中TRB3和Akt mRNA的錶達水平。結果:妊娠、高脂高糖飲食均可緻大鼠HOMA-IR升高( F妊娠=2318.491,F飲食=2888.237,F交互=993.094, P均<0.001),同時大鼠肝組織中TRB3 mRNA的錶達水平升高(F妊娠=256.887,F飲食=1749.406,F交互=2.579, P均<0.001),Akt mRNA的錶達水平降低(F妊娠=221.091,F飲食=1416.984,F交互=28.918,P均<0.001)。高脂高糖妊娠組、榦預組和普通飲食妊娠組HOMA-IR、TRB3和Akt mRNA的錶達水平差異均有統計學意義( F=2772.745、445.896和390.334,P均<0.001);榦預組HOMA-IR、TRB3 mRNA的錶達水平較高脂高糖妊娠組降低,但仍高于普通飲食妊娠組,Akt mRNA的錶達水平較高脂高糖妊娠組升高,但仍低于普通飲食妊娠組(P<0.05)。結論:TRB3和Akt可能參與瞭妊娠期胰島素牴抗的髮生髮展,飲食榦預可改善TRB3/Akt通路,減輕妊娠期胰島素牴抗。
목적:관찰음식간예대고지고당임신대서간조직중 Tribbles동원단백3(TRB3)화Akt mRNA적표체급이도소저항적영향。방법:75지건강자성SD대서수궤분위고지고당임신조、고지고당비임신조、보통음식임신조、보통음식비임신조급고지고당음식임신후보통음식간예조(간예조)5조,매조15지。임신제20천,용전자동생화검측의검측FBG,ELISA시제합측정FINS,유차계산HOMA-IR。응용RT-PCR측정대서간조직중TRB3화Akt mRNA적표체수평。결과:임신、고지고당음식균가치대서HOMA-IR승고( F임신=2318.491,F음식=2888.237,F교호=993.094, P균<0.001),동시대서간조직중TRB3 mRNA적표체수평승고(F임신=256.887,F음식=1749.406,F교호=2.579, P균<0.001),Akt mRNA적표체수평강저(F임신=221.091,F음식=1416.984,F교호=28.918,P균<0.001)。고지고당임신조、간예조화보통음식임신조HOMA-IR、TRB3화Akt mRNA적표체수평차이균유통계학의의( F=2772.745、445.896화390.334,P균<0.001);간예조HOMA-IR、TRB3 mRNA적표체수평교고지고당임신조강저,단잉고우보통음식임신조,Akt mRNA적표체수평교고지고당임신조승고,단잉저우보통음식임신조(P<0.05)。결론:TRB3화Akt가능삼여료임신기이도소저항적발생발전,음식간예가개선TRB3/Akt통로,감경임신기이도소저항。
Aim: To study the effects of dietary intervention on the expressions of tribbles homologous protein 3 ( TRB3) and Akt mRNA in hepatic tissue and insulin resistance ( IR) of pregnant rats with high sucrose and fat diet .Meth-ods:Seventy-five healthy female SD rats were divided into five groups randomly: high sucrose and fat diet pregnant rat (HP) group,high sucrose and fat diet virgin rat(HV) group,normal diet pregnant rat(NP) group, normal diet virgin rat (NV) group and dietary intervention pregnant rat (GHP) group.Twenty days after gestation,fasting plasma glucose and fasting insulin were measured ,and IR index ( HOMA-IR) was calculated accordingly .RT-PCR was performed to detect the expressions of TRB3 and Akt mRNA in hepatic tissue .Results:Gestation and high sucrose and fat diet could cause the in-crease of HOMA-IR and TRB3 mRNA( HOMA-IR:Fgestation =2 318.491,Fdiet =2 888.237, Finteraction =993.094,all P<0.001;TRB3 mRNA:Fgestation =256.887,Fdiet =1 749.406, Finteraction =2.579,all P<0.001),and the decrease of Akt mRNA(Fgestation =221.091,Fdiet =1 416.984, Finteraction =28.918,all P<0.001).HOMA-IR and the levels of TRB3 mR-NA and Akt mRNA among HP group , NP group and GHP group had significant differences .HOMA-IR and the level of TRB3 mRNA in GHP group were lower than those of HP group , but still higher than those of NP group; the level of Akt mRNA was higher than that of HP group , but still lower than that of NP group (all P<0.05).Conclusion:TRB3 and Akt might be involved in the IR in gestation .Dietary intervention could decrease IR by improving TRB 3/Akt signal path .