CAJ | 학술논문

目的：评价结核分枝杆菌复合抗原ESAT-6/CFP-10/Rv0315在活动性肺结核诊断中的应用价值。方法：选取初治肺结核病患者70例和健康体检者34例作为结核组和健康对照组，分离PBMCs，分别用复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库作为抗原进行ELISPOT实验，检测斑点形成细胞数。结果：复合抗原ESAT-6/CFP-10/Rv0315、ESAT-6肽段库和CFP-10肽段库检测结核分枝杆菌感染的阳性率依次为85．7％（60/70）、77．1％（54/70）、72．9％（51/70），特异性依次为82．4％（28/34）、85．3％（29/34）、88．2％（30/34）。结论：复合抗原ESAT-6/CFP-10/Rv0315作为抗原建立的ELISPOT 检测方法在活动性肺结核的辅助诊断中有较高的应用价值。
목적：평개결핵분지간균복합항원ESAT-6/CFP-10/Rv0315재활동성폐결핵진단중적응용개치。방법：선취초치폐결핵병환자70례화건강체검자34례작위결핵조화건강대조조，분리PBMCs，분별용복합항원ESAT-6/CFP-10/Rv0315、ESAT-6태단고화CFP-10태단고작위항원진행ELISPOT실험，검측반점형성세포수。결과：복합항원ESAT-6/CFP-10/Rv0315、ESAT-6태단고화CFP-10태단고검측결핵분지간균감염적양성솔의차위85．7％（60/70）、77．1％（54/70）、72．9％（51/70），특이성의차위82．4％（28/34）、85．3％（29/34）、88．2％（30/34）。결론：복합항원ESAT-6/CFP-10/Rv0315작위항원건립적ELISPOT 검측방법재활동성폐결핵적보조진단중유교고적응용개치。
Aim:To evaluate the application value of multiple antigens ESAT-6/CFP-10/Rv0315 in the detection of active pulmonary tuberculosis .Methods:A total of 70 patients with initial pulmonary tuberculosis and 34 healthy persons were enrolled as tuberculosis group and control group .PBMCs isolated from two groups were co-cultured with ESAT-6/CFP-10/Rv0315 , ESAT-6 and CFP-10 antigens .SFCs were enumerated by ELISPOT .Results: The sensitivity of ESAT-6/CFP-10/Rv0315, ESAT6 and CFP-10 antigens by ELISPOT was 85.7%(60/70), 77.1%(54/70), and 72.9%(51/70), respectively.Specificity was 82.4%(28/34), 85.3%(29/34), and 88.2%(30/34), respectively.Conclusion:Multiple antigens ESAT-6/CFP-10/Rv0315 showed certain value for auxiliary diagnosis of active pulmonary tuberculosis .