CAJ | 학술논문

目的：观察不同含量常压氧（ NBO）对急性脑缺血再灌注大鼠脑组织Caspase-9及Caspase-3 mRNA表达的影响。方法：选取制作脑缺血再灌注损伤模型成功的120只SPF级雄性SD大鼠，分为低、中、高含量NBO组和空气组，在持续吸氧或空气3、6、12、24、48、72 h时，各组分别随机抽取5只大鼠处死，断头取脑，用荧光定量PCR检测Caspase-9及Caspase-3 mRNA的表达水平。结果：空气组和低、中、高含量NBO组Caspase-9及Caspase-3 mRNA的表达在48 h达到高峰，之后开始下降（ P均＜0．05），且各时间点NBO组均低于空气组（ P均＜0．05）。结论：NBO可以下调急性脑缺血再灌注后脑组织Caspase-9及Caspase-3 mRNA的表达。
목적：관찰불동함량상압양（ NBO）대급성뇌결혈재관주대서뇌조직Caspase-9급Caspase-3 mRNA표체적영향。방법：선취제작뇌결혈재관주손상모형성공적120지SPF급웅성SD대서，분위저、중、고함량NBO조화공기조，재지속흡양혹공기3、6、12、24、48、72 h시，각조분별수궤추취5지대서처사，단두취뇌，용형광정량PCR검측Caspase-9급Caspase-3 mRNA적표체수평。결과：공기조화저、중、고함량NBO조Caspase-9급Caspase-3 mRNA적표체재48 h체도고봉，지후개시하강（ P균＜0．05），차각시간점NBO조균저우공기조（ P균＜0．05）。결론：NBO가이하조급성뇌결혈재관주후뇌조직Caspase-9급Caspase-3 mRNA적표체。
Aim:To explore the effects of different concentrations of normobaric oxygen ( NBO ) on Caspase-9 and Caspase-3 mRNA expression in brain tissue of rats after acute cerebral ischemia-reperfusion .Methods:A total of 120 SPF level male SD rats were subjected to middle cerebral artery occlusion-reperfusion model .The rats were randomly divided in-to four groups , of which 3 groups ( n=90 ) were given three concentrations of NBO treatment respectively and the other group was the air group (n=30).When taking persistent oxygen or air for 3, 6, 12, 24, 48, 72 h, five rats randomly taken in each group would be sacrificed , with brain collected .Fluorescence quantitative PCR was used to detect Caspase-9 and Caspase-3 mRNA expression levels .Results:The expression of Caspase-9 and Caspase-3 mRNA peaked in air group and low, medium, high concentration of NBO treatment groups at 48 h, then began to decline(P<0.05), and at each time point those of the NBO groups were lower than that of air group (P<0.05).Conclusion:NBO could reduce Caspase-9 and Caspase-3 mRNA expression in cerebral ischemia-reperfusion.