CAJ | 학술논문

目的：观察绞股蓝总皂苷（ Gyp）对光老化人皮肤成纤维细胞株HSF凋亡的影响。方法：分别按80、160和320 mg/d的剂量灌胃大鼠Gyp生理盐水溶液，7 d后取血，分离血清备用。采用长波紫外线（ UVA）照射方法建立光老化HSF细胞模型，照射剂量36 J/cm2，然后用制备的低、中、高剂量Gyp含药血清进行干预培养。12 h后，采用TUNEL法检测细胞凋亡情况，采用RT-PCR和Western blot法检测细胞中Bax、Bcl-2 mRNA和蛋白的表达。以未照射细胞（空白对照组）、模型细胞和正常血清干预的模型细胞（正常血清组）作对照。结果：6组凋亡细胞数，细胞中Bcl-2、Bax mRNA和蛋白的表达水平差异均有统计学意义（ F＝474．201，351．094、334．907和403．610、162．766， P＜0．001）。与空白对照组比较，UVA模型组和正常血清组细胞凋亡增强，Bcl-2 mRNA和蛋白表达降低，同时Bax mRNA和蛋白表达升高（P＜0．05）。与UVA模型组和正常血清组比较，低、中、高剂量Gyp组细胞凋亡减弱，细胞中Bcl-2 mRNA和蛋白表达升高，Bax mRNA和蛋白表达降低（P＜0．05），高剂量组效果更显著（P＜0．05）。结论：Gyp可以逆转UVA诱导的人皮肤成纤维细胞的凋亡。
목적：관찰교고람총조감（ Gyp）대광노화인피부성섬유세포주HSF조망적영향。방법：분별안80、160화320 mg/d적제량관위대서Gyp생리염수용액，7 d후취혈，분리혈청비용。채용장파자외선（ UVA）조사방법건립광노화HSF세포모형，조사제량36 J/cm2，연후용제비적저、중、고제량Gyp함약혈청진행간예배양。12 h후，채용TUNEL법검측세포조망정황，채용RT-PCR화Western blot법검측세포중Bax、Bcl-2 mRNA화단백적표체。이미조사세포（공백대조조）、모형세포화정상혈청간예적모형세포（정상혈청조）작대조。결과：6조조망세포수，세포중Bcl-2、Bax mRNA화단백적표체수평차이균유통계학의의（ F＝474．201，351．094、334．907화403．610、162．766， P＜0．001）。여공백대조조비교，UVA모형조화정상혈청조세포조망증강，Bcl-2 mRNA화단백표체강저，동시Bax mRNA화단백표체승고（P＜0．05）。여UVA모형조화정상혈청조비교，저、중、고제량Gyp조세포조망감약，세포중Bcl-2 mRNA화단백표체승고，Bax mRNA화단백표체강저（P＜0．05），고제량조효과경현저（P＜0．05）。결론：Gyp가이역전UVA유도적인피부성섬유세포적조망。
Aim:To investigate the effect of gypenosides ( Gyp) on the apoptosis of photoaging human dermal fibro-blasts(HSF cells) induced by ultraviolet A(UVA) radiation.Methods:Rats were intragastricly administrated Gyp saline solution at doses of 80, 160 and 320 mg/d, respectively.7 d later, the serum was isolated.HSF cells were irradiated by UVA at a dose of 35 mJ/cm2 to establish photoaging cell model ,then these cells were cultured by low-,median-and high-dose Gyp medicated serum(Gyp groups).12 h later, apoptosis was detected by TUNEL , the expressions of Bax and Bcl-2 mRNA and protein in cells were detected using RT-PCR and Western blot ,respectively .The cells without irradiation ( blank control group), model cells without intervention(model group) and with normal serum intervention (normal serum group) were the controls.Results: There were significant differences in apoptosis cell number ,expressions of Bcl-2 mRNA,Bax mRNA,Bcl-2 and Bax protein in cells among the six groups (F=474.201,351.094,334.907 and 403.610,162.766,P<0.001).Compared with the blank control group , apoptosis of model group and normal serum group increased , and the ex-pressions of Bcl-2 mRNA and protein decreased,while those of Bax increased significantly (P<0.05).Compared with the model group and normal serum group , apoptosis of Gyp groups decreased ,and the expressions of Bcl-2 mRNA and protein increased,while those of Bax decreased significantly (P<0.05),especially the high-dose Gyp group(P<0.05).Conclu-sion:Gyp can protect HSF cells from UVA-induced apoptosis .