郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
2期
169-172
,共4页
何光耀%谢貌%唐安洲%谭颂华
何光耀%謝貌%唐安洲%譚頌華
하광요%사모%당안주%담송화
苦参碱%鼻咽癌%凋亡%移植瘤%裸鼠
苦參堿%鼻嚥癌%凋亡%移植瘤%裸鼠
고삼감%비인암%조망%이식류%라서
matrine%nasopharyngeal carcinoma%apoptosis%xenograft%nude mice
目的：观察苦参碱对人鼻咽癌CNE2细胞裸鼠移植瘤生长及凋亡相关基因表达的影响。方法：20只裸鼠皮下接种CNE2，建立移植瘤模型，成瘤后，随机分成4组，每组5只。苦参碱高、低剂量组分别腹腔注射100、50 mg/kg苦参碱，阳性对照组腹腔注射顺铂2 mg/kg，阴性对照组给予生理盐水。给药7次后，应用体内抑瘤实验测定抑瘤率，分别采用real-time PCR法和Western blot法检测移植瘤组织中bax、bcl-2、caspase-3 mRNA和蛋白的表达。结果：阳性对照组和苦参碱低、高剂量组抑瘤率分别为51．6％和26．4％、43．2％。4组间移植瘤组织中bax、bcl-2、caspase-3的mRNA和蛋白表达差异有统计学意义（F＝3．751、16．113、3．918和26．500、9．771、12．255，P＜0．05）；与阴性对照组比较，阳性对照组和苦参碱高、低剂量组bax mRNA 和蛋白表达及阳性对照组、苦参碱高剂量组caspase-3 mRNA和蛋白表达上调（P＜0．05），bcl-2 mRNA和蛋白表达下调（P＜0．05）。结论：苦参碱可通过调节CNE2移植瘤组织中凋亡相关基因的表达抑制肿瘤的生长。
目的：觀察苦參堿對人鼻嚥癌CNE2細胞裸鼠移植瘤生長及凋亡相關基因錶達的影響。方法：20隻裸鼠皮下接種CNE2，建立移植瘤模型，成瘤後，隨機分成4組，每組5隻。苦參堿高、低劑量組分彆腹腔註射100、50 mg/kg苦參堿，暘性對照組腹腔註射順鉑2 mg/kg，陰性對照組給予生理鹽水。給藥7次後，應用體內抑瘤實驗測定抑瘤率，分彆採用real-time PCR法和Western blot法檢測移植瘤組織中bax、bcl-2、caspase-3 mRNA和蛋白的錶達。結果：暘性對照組和苦參堿低、高劑量組抑瘤率分彆為51．6％和26．4％、43．2％。4組間移植瘤組織中bax、bcl-2、caspase-3的mRNA和蛋白錶達差異有統計學意義（F＝3．751、16．113、3．918和26．500、9．771、12．255，P＜0．05）；與陰性對照組比較，暘性對照組和苦參堿高、低劑量組bax mRNA 和蛋白錶達及暘性對照組、苦參堿高劑量組caspase-3 mRNA和蛋白錶達上調（P＜0．05），bcl-2 mRNA和蛋白錶達下調（P＜0．05）。結論：苦參堿可通過調節CNE2移植瘤組織中凋亡相關基因的錶達抑製腫瘤的生長。
목적：관찰고삼감대인비인암CNE2세포라서이식류생장급조망상관기인표체적영향。방법：20지라서피하접충CNE2，건립이식류모형，성류후，수궤분성4조，매조5지。고삼감고、저제량조분별복강주사100、50 mg/kg고삼감，양성대조조복강주사순박2 mg/kg，음성대조조급여생리염수。급약7차후，응용체내억류실험측정억류솔，분별채용real-time PCR법화Western blot법검측이식류조직중bax、bcl-2、caspase-3 mRNA화단백적표체。결과：양성대조조화고삼감저、고제량조억류솔분별위51．6％화26．4％、43．2％。4조간이식류조직중bax、bcl-2、caspase-3적mRNA화단백표체차이유통계학의의（F＝3．751、16．113、3．918화26．500、9．771、12．255，P＜0．05）；여음성대조조비교，양성대조조화고삼감고、저제량조bax mRNA 화단백표체급양성대조조、고삼감고제량조caspase-3 mRNA화단백표체상조（P＜0．05），bcl-2 mRNA화단백표체하조（P＜0．05）。결론：고삼감가통과조절CNE2이식류조직중조망상관기인적표체억제종류적생장。
Aim:To investigate the effect of matrine on expressions of apoptosis-related genes and growth of human na-sopharyngeal carcinoma CNE 2 cell xenografts in nude mice .Methods:A total of 20 nude mice were subcutaneously inocu-lated with CNE2.After the successful establishment of xenograft model ,the 20 nude mice were randomly divided into 4 groups, 5 rats in each group .Low-and high-dose matrine groups were intraperitoneally injected with matrine at dose of 50 and 100 mg/kg, positive control group was injected with cisplatin ,and negative control group received normal saline .After 7 times administration , the tumor inhibition rate was detected , and the mRNA and protein expressions of bax , bcl-2 and caspase-3 were detected by real-time PCR and Western blot,respectively.Results: The tumor inhibition rates of positive control group, and low-,high-dose matrine groups were 51.6%, 26.4%, and 43.2%;the mRNA and protein expressions of bax,bcl-2 and caspase-3 in xenograft tissue had significant differences (F=3.751,16.113,3.918 and 26.500,9.771, 12.255, P<0.05).Compared with the negative control group , the mRNA and protein expressions of bax in positive con-trol group and low-,high-dose matrine groups and those of caspase-3 in positive control group and high-dose matrine group increased(P<0.05),but those of bcl-2 decreased(P<0.05).Conclusion: Matrine could inhibit the growth of CNE2 xenograft through regulating the expressions of apoptosis-related genes .
