CAJ | 학술논문

采用蛋白酶法与酸碱法提取鼠妇蛋白多肽成分,利用化学刺激和热刺激法观察提取物对小鼠的镇痛作用,同时测定小鼠脑组织中的去甲肾上腺素(NE),多巴胺(DA)和5羟色胺(5-HT)水平。以蛋白多肽中氨基酸种类,尤其是兴奋性氨基酸天门冬氨酸和谷氨酸,抑制性氨基酸甘氨酸和γ氨基丁酸的质量分数为指标,醋酸扭体次数抑制率和热板实验痛阈值为镇痛活性指标,对两种提取方法进行比较并且初步研究镇痛作用机理,结果表明酸碱法制备的富含蛋白多肽的鼠妇提取物镇痛效果优于酶法,该提取物能明显提高小鼠醋酸扭体次数抑制率与热板实验痛阈值,且能明显提高小鼠脑内NE,DA和5-HT质量分数水平。酸碱法提取蛋白多肽对小鼠具有明显的镇痛作用,其镇痛作用可能与脑内的单胺递质类5-HT有关。
채용단백매법여산감법제취서부단백다태성분,이용화학자격화열자격법관찰제취물대소서적진통작용,동시측정소서뇌조직중적거갑신상선소(NE),다파알(DA)화5간색알(5-HT)수평。이단백다태중안기산충류,우기시흥강성안기산천문동안산화곡안산,억제성안기산감안산화γ안기정산적질량분수위지표,작산뉴체차수억제솔화열판실험통역치위진통활성지표,대량충제취방법진행비교병차초보연구진통작용궤리,결과표명산감법제비적부함단백다태적서부제취물진통효과우우매법,해제취물능명현제고소서작산뉴체차수억제솔여열판실험통역치,차능명현제고소서뇌내NE,DA화5-HT질량분수수평。산감법제취단백다태대소서구유명현적진통작용,기진통작용가능여뇌내적단알체질류5-HT유관。
Polypeptide rich fractions have been prepared by proteolytic enzymes and 1 mol/L HCl &1 mol/L NaOH extraction.Analgesic activities of Armadillidiumvulgare extracts have been evaluated by mice pain model induced by injection of acetic acid and hot plate.The level of NE,DA and 5-HT in mice brain has been measured by HPLC method with Fluorescence detector.The content and type of amino acid,spe-cifically excitatory amino acid asparitic acid,glutamic acid and inhibitory amino acid glycine,γ-aminobutyr-ic acid as index,acetic acid and hot plate as analgesic effect index.The results show that polypeptide rich fraction of 1mol/L HCl &1 mol/L NaOH method can significantly inhibited the body writhing count and increased the pain thresholds of mice in hot plate test.The level of NE,DA and 5-HT is significantly in-creased in mice.The 1mol/L HCl &1 mol/L NaOH extraction is showed to be the most promising method for extracting polypeptide from Armadillidiumvulgare.Polypeptide rich fraction of Armadilllium vulgare extract had significant analgesic effects in mice and the analgesic effect may due to the increase of 5-HT in mice brain.