贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
3期
136-139
,共4页
毛木耳%液体发酵%培养基%培养条件%优化
毛木耳%液體髮酵%培養基%培養條件%優化
모목이%액체발효%배양기%배양조건%우화
Auricularia polytricha%liquid fermentation%medium%culture condition%optimization
为满足市场需要，缩短毛木耳的生产周期，采用单因子试验和正交试验对毛木耳988菌种液体发酵培养基中碳、氮源的种类及浓度和发酵条件进行优化。结果表明：毛木耳液体发酵培养基的最佳组成为马铃薯20％，蔗糖2％，玉米粉1．5％，蛋白胨0．6％，KH2 PO40．1％，MgSO4·7H 2 O 0．02％；最佳发酵培养条件为装液量90 mL/250mL，摇床转速160 r/min，此时可获最大菌丝球平均干重，得率达1．5173 g/100mL。
為滿足市場需要，縮短毛木耳的生產週期，採用單因子試驗和正交試驗對毛木耳988菌種液體髮酵培養基中碳、氮源的種類及濃度和髮酵條件進行優化。結果錶明：毛木耳液體髮酵培養基的最佳組成為馬鈴藷20％，蔗糖2％，玉米粉1．5％，蛋白胨0．6％，KH2 PO40．1％，MgSO4·7H 2 O 0．02％；最佳髮酵培養條件為裝液量90 mL/250mL，搖床轉速160 r/min，此時可穫最大菌絲毬平均榦重，得率達1．5173 g/100mL。
위만족시장수요，축단모목이적생산주기，채용단인자시험화정교시험대모목이988균충액체발효배양기중탄、담원적충류급농도화발효조건진행우화。결과표명：모목이액체발효배양기적최가조성위마령서20％，자당2％，옥미분1．5％，단백동0．6％，KH2 PO40．1％，MgSO4·7H 2 O 0．02％；최가발효배양조건위장액량90 mL/250mL，요상전속160 r/min，차시가획최대균사구평균간중，득솔체1．5173 g/100mL。
In order to meet the needs of the market and shorten the production cycle of A.polytricha, the liquid fermentation medium including the kinds and concentrations of carbon and nitrogen source,and fermentation conditions of A.polytricha 988 was studied through the single factor experiment and orthogonal experiment design.Results:The best liquid fermentation medium components included 20%potato,2% sucrose,1.5% corn flour,0.6% peptone,0.1% KH2 PO4 ,0.02% MgSO4 7H 2 O;The best culture conditions was as follows:the volume of liquid ratio 90 mL/250mL,the shaking speed 160 r/min. Under these conditions,the maximum average dry weight of mycelium could be got,the yield might be up to 1.5173 g/ 100 mL.
