CAJ | 학술논문

为了获得较高的蝉花核苷碱基，为其品质评估提供方法，采用超声波法对蝉花中的核苷和碱基进行提取，通过单因素和正交试验确定最优提取条件，运用高效液相色谱法对提取液中的8种核苷碱基类物质进行分析测定。结果表明：最优提取条件是以纯水为提取溶剂，料液比1∶40，提取液 pH 4，超声提取温度60℃，提取90 min，在该条件下的总核苷和碱基的提取得率可达2．74％。
위료획득교고적선화핵감감기，위기품질평고제공방법，채용초성파법대선화중적핵감화감기진행제취，통과단인소화정교시험학정최우제취조건，운용고효액상색보법대제취액중적8충핵감감기류물질진행분석측정。결과표명：최우제취조건시이순수위제취용제，료액비1∶40，제취액 pH 4，초성제취온도60℃，제취90 min，재해조건하적총핵감화감기적제취득솔가체2．74％。
In order to gain more nucleosides and bases from C.ciecadae and provide a method to evaluate its quality,the nucleosides and bases were extracted from C.ciecadae by ultrasonic method, which was optimized through a single-factor test and an orthogonal test.Then eight kinds of nucleosides and bases extracted from C.ciecadae were analyzed and detected by HPLC method.The results showed that the optimum extraction conditions included pure water as the solvent,solid-liquid ratio 1:40,pH 4, extraction temperature 60℃ and extraction time 90 min.Under these conditions,the extraction rate of total nucleosides and bases could reach 2.74%.