CAJ | 학술논문

为探明苦荞秸秆中总黄酮类物质提取工艺及其抗氧化性，为其进一步的开发利用提供依据，以苦荞秸秆为原料，采用单因素与正交试验结合，研究了微波萃取温度、萃取时间、乙醇浓度、料液比等工艺参数对总黄酮得率的影响。结果表明：苦荞秸秆中总黄酮的最佳微波萃取工艺为萃取温度80℃、乙醇浓度65％、料液比1∶35、萃取时间20 min，在该条件下的提取率为1．53％。苦荞秸秆总黄酮的抗氧化性随着浓度的升高而增强，当总黄酮浓度为1．55μg/mL 时，羟基自由基清除率达71．6％，当总黄酮浓度为9．94μg/mL 时，对 DPPH 自由基的清除率为85．7％。
위탐명고교갈간중총황동류물질제취공예급기항양화성，위기진일보적개발이용제공의거，이고교갈간위원료，채용단인소여정교시험결합，연구료미파췌취온도、췌취시간、을순농도、료액비등공예삼수대총황동득솔적영향。결과표명：고교갈간중총황동적최가미파췌취공예위췌취온도80℃、을순농도65％、료액비1∶35、췌취시간20 min，재해조건하적제취솔위1．53％。고교갈간총황동적항양화성수착농도적승고이증강，당총황동농도위1．55μg/mL 시，간기자유기청제솔체71．6％，당총황동농도위9．94μg/mL 시，대 DPPH 자유기적청제솔위85．7％。
The technology of extraction of total flavonoids in tartary buckwheat straw and it’s antioxidant activities were studied to provide a reference for its further utilization and exploitation,taking tartary buckwheat straw as the material,the effects of temperature,time,ethanol concentration and solid-liquid ratio on the extraction of total flavonoids from tartary buckwheat straw were investigated using the methods of single factor test and orthogonal design test of L9 (3 4 ).The results showed that the highest extraction rate (1.53%) of total flavonoids could be obtained under conditions of 65% ethanol, temperature 80℃,solid-liquid ratio 1 ∶35 and extraction time 20 min.The antioxidant property of total flavonoids increased with flavonoids concentration increasing.Hydroxy radical scavenging rate was 71.6%when total flavonoids concentration was 1.55 g/mL and DPPH free radical scavenging rate was 85.7%when total flavonoids concentration was 9.94 g/mL.