贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
3期
112-116
,共5页
刘畅%罗志宇%冉雪琴%王嘉福
劉暢%囉誌宇%冉雪琴%王嘉福
류창%라지우%염설금%왕가복
microRNAs%miR-1%miR-133%miR-206%贵州地方猪种%实时荧光定量 PCR
microRNAs%miR-1%miR-133%miR-206%貴州地方豬種%實時熒光定量 PCR
microRNAs%miR-1%miR-133%miR-206%귀주지방저충%실시형광정량 PCR
microRNAs%miR-1%miR-133%miR-206%Guihzhou indigenous pig%real-time PCR
为探明 miR-1、miR-133、miR-206基因在不同品种猪肌肉组织中的表达及其与屠宰性能间的关系，以6月龄大白猪、糯谷猪、关岭猪及黔南黑猪的背最长肌为材料，采用实时荧光定量 PCR 方法，研究了3种 microRNAs（miRNAs）在贵州地方猪种和大白猪背最长肌中的表达差异。结果表明：成年期 miR-133的表达量高于另外2种 miRNA 分子。miR-133在糯谷猪中的表达量是大白猪的1．58倍（P ＜0．05），与其眼肌面积、花油重高度负相关，与屠宰率高度正相关。miR-206在糯谷猪中的表达量是大白猪的2．23倍（P ＜0．05），与其瘦肉率高度负相关，与板油重中度正相关。在关岭猪背最长肌中 miR-133的表达量最高，是大白猪的2．67倍（P ＜0．05），miR-133和 miR-206的表达量与眼肌面积、胴体重高度正相关，与花油重、板油重和脂肪比负相关；但 miR-206的表达量明显低于其他猪种。不同品种猪中 miR-133和 miR-206存在差异表达，对地方猪品种的骨骼肌生长和脂肪沉积有一定的调节作用。
為探明 miR-1、miR-133、miR-206基因在不同品種豬肌肉組織中的錶達及其與屠宰性能間的關繫，以6月齡大白豬、糯穀豬、關嶺豬及黔南黑豬的揹最長肌為材料，採用實時熒光定量 PCR 方法，研究瞭3種 microRNAs（miRNAs）在貴州地方豬種和大白豬揹最長肌中的錶達差異。結果錶明：成年期 miR-133的錶達量高于另外2種 miRNA 分子。miR-133在糯穀豬中的錶達量是大白豬的1．58倍（P ＜0．05），與其眼肌麵積、花油重高度負相關，與屠宰率高度正相關。miR-206在糯穀豬中的錶達量是大白豬的2．23倍（P ＜0．05），與其瘦肉率高度負相關，與闆油重中度正相關。在關嶺豬揹最長肌中 miR-133的錶達量最高，是大白豬的2．67倍（P ＜0．05），miR-133和 miR-206的錶達量與眼肌麵積、胴體重高度正相關，與花油重、闆油重和脂肪比負相關；但 miR-206的錶達量明顯低于其他豬種。不同品種豬中 miR-133和 miR-206存在差異錶達，對地方豬品種的骨骼肌生長和脂肪沉積有一定的調節作用。
위탐명 miR-1、miR-133、miR-206기인재불동품충저기육조직중적표체급기여도재성능간적관계，이6월령대백저、나곡저、관령저급검남흑저적배최장기위재료，채용실시형광정량 PCR 방법，연구료3충 microRNAs（miRNAs）재귀주지방저충화대백저배최장기중적표체차이。결과표명：성년기 miR-133적표체량고우령외2충 miRNA 분자。miR-133재나곡저중적표체량시대백저적1．58배（P ＜0．05），여기안기면적、화유중고도부상관，여도재솔고도정상관。miR-206재나곡저중적표체량시대백저적2．23배（P ＜0．05），여기수육솔고도부상관，여판유중중도정상관。재관령저배최장기중 miR-133적표체량최고，시대백저적2．67배（P ＜0．05），miR-133화 miR-206적표체량여안기면적、동체중고도정상관，여화유중、판유중화지방비부상관；단 miR-206적표체량명현저우기타저충。불동품충저중 miR-133화 miR-206존재차이표체，대지방저품충적골격기생장화지방침적유일정적조절작용。
To envelope the relationship of miRNAs and muscle growth in pig,the longissimus dorsi muscle were sampled from three indigenous pig breeds,including Nuogu pig,Guanling pig,Qiannan Black pig in 6 months old,taking Yorkshire pig as the control.The expression levels of miR-1,miR-133,miR-206 were analyzed by real-time quantitative PCR detecting system(qPCR)with stem-loop primer for reverse transcription of miRNAs.The results showed that the expression level of miR-133 was higher than both of miR-1 and miR-206 in the longissimus dorsi muscle of pigs.Further,miR-133 level in Nuogu pig was 1.58 times higher than that in Yorkshire pig (P<0.05).miR-133 level was negatively correlated with the eye muscle area and abdominal fat weight,while positively correlated with the slaughter of Nuogu pig breed. miR-206 level in Nuogu pig was 2.23 times higher than that in Yorkshire pig (P <0.05),and negatively correlated with the leanness,positively correlated with the leaf fat weight of Nuogu pig.However,the trends of miRNAs change in Guanling pig breed were much different from the others.The expression level of miR-133 was the highest but miR-206 level was the lowest in Guanling pig with 2.67 times higher in miR-133.The levels in both of miR-133 and miR-206 were positively correlated with the eye muscle area and carcass weight of Guanling pig,and negatively correlated with abdominal fat weight,leaf fat weight and fat ratio.miR-133 and miR-206 in different pig breeds had differential expression,which regulated the body growth and fat deposition in indigenous pig breeds of Guizhou Province in certain degree.
