CAJ | 학술논문

为半夏建立有效生物防治和高产优质的绿色种植，采用研磨法和组织块培养法从贵州健康半夏植株的叶、叶柄、块茎中分离纯化到8株内生细菌，并采用细菌16SrDNA 序列分析的分子生物学方法结合传统形态和生理生化鉴定方法进行种属鉴定。结果表明：8株内生细菌被鉴定为5属6个种，即芽孢杆菌属的嗜碱芽孢杆菌（Bacillus alcalophilus ）、微杆菌属的产左聚糖微杆菌（Microbacterium laevanifor-mans ）、短芽孢杆菌属的土壤短芽孢杆菌（Brevibacillus agri ）和热红短芽孢杆菌（Brevibacillus thermoru-ber ）、寡养单胞菌属的嗜麦芽寡养单胞菌（Stenotrophomonas maltrophilia ）、类芽孢杆菌属的多黏类芽孢杆菌（Paenibacillus polymyxa ）。说明，贵州半夏体内含有多种内生细菌，多数为杆菌，其中芽孢杆菌、多黏类芽孢杆菌等将为半夏益生菌的开发奠定基础。
위반하건립유효생물방치화고산우질적록색충식，채용연마법화조직괴배양법종귀주건강반하식주적협、협병、괴경중분리순화도8주내생세균，병채용세균16SrDNA 서렬분석적분자생물학방법결합전통형태화생리생화감정방법진행충속감정。결과표명：8주내생세균피감정위5속6개충，즉아포간균속적기감아포간균（Bacillus alcalophilus ）、미간균속적산좌취당미간균（Microbacterium laevanifor-mans ）、단아포간균속적토양단아포간균（Brevibacillus agri ）화열홍단아포간균（Brevibacillus thermoru-ber ）、과양단포균속적기맥아과양단포균（Stenotrophomonas maltrophilia ）、류아포간균속적다점류아포간균（Paenibacillus polymyxa ）。설명，귀주반하체내함유다충내생세균，다수위간균，기중아포간균、다점류아포간균등장위반하익생균적개발전정기출。
To build a green planting pattern of P .ternata with effective biological control and high yield and quality, eight endophytic strains were isolated and purified by grinding and tissure block culturing methods from tubers,leaves and stipe of health P .ternata in Guizhou.After purification and culturing,endophytic bacteria were identified by 16S rDNA gene (for bacteria)based molecular biology method association with morphological observation and physiological biochemical reactions.The results showed that eight endophytic bacterias were classified into five genera of six families,Bacillus alcalophilus , Microbacterium laevaniformans , Brevibacillus agri , Brevibacillus thermoruber , Stenotrophomonas maltrophilia and Paenibacillus polymyxa . This indicated that various endophytic species existed in P .ternate,and most of them were bacilus.Bacillus sp and Paenibacillus polymyxa isolated from P .ternata would contribute to preparing the probiotic preparations for P .ternata.