CAJ | 학술논문

为探明外源 Ca2＋对药用植物栝楼抗寒性的变化，以耐寒性较弱的安庆栝楼和耐寒性较强的三门峡栝楼为对比试验材料，采用营养液栽培法，研究了外源 Ca2＋对药用植物栝楼抗寒机制的影响。结果表明：栝楼在低温胁迫下喷施不同浓度的外源 Ca2＋后，Pro 含量和 POD 活性均随 Ca2＋浓度的增大呈先上升再逐渐下降趋势，PPO 活性均随 Ca2＋浓度的增大呈先下降后逐渐上升的趋势，Ca2＋浓度为5 mmol/L 时，两个栝楼品种 Pro 含量、POD 活性与对照组相比均具有显著性差异（P ＜0．05），其中 Pro 含量分别比对照组提高71．3％和33．0％，POD 活性分别比对照组高244．2％和253．0％；以相同浓度（5 mmol/L）的外源 Ca2＋诱导喷施不同时间后，对低温胁迫下的栝楼具有不同的低温缓解效应，其中在诱导喷施时长为3 d 时具有较为显著的低温缓解效应。
위탐명외원 Ca2＋대약용식물괄루항한성적변화，이내한성교약적안경괄루화내한성교강적삼문협괄루위대비시험재료，채용영양액재배법，연구료외원 Ca2＋대약용식물괄루항한궤제적영향。결과표명：괄루재저온협박하분시불동농도적외원 Ca2＋후，Pro 함량화 POD 활성균수 Ca2＋농도적증대정선상승재축점하강추세，PPO 활성균수 Ca2＋농도적증대정선하강후축점상승적추세，Ca2＋농도위5 mmol/L 시，량개괄루품충 Pro 함량、POD 활성여대조조상비균구유현저성차이（P ＜0．05），기중 Pro 함량분별비대조조제고71．3％화33．0％，POD 활성분별비대조조고244．2％화253．0％；이상동농도（5 mmol/L）적외원 Ca2＋유도분시불동시간후，대저온협박하적괄루구유불동적저온완해효응，기중재유도분시시장위3 d 시구유교위현저적저온완해효응。
Anqing T.kirilowii with weak cold resistance and Sanmenxia T.kirilowii with strong cold resistance were cultured by hydroponics to study the effect of exogenous Ca2+ on cold resistance of T.kirilowii.The PRO content and POD activity of two T.kirilowii varieties sprayed exogenous Ca2+with different concentration under low temperature stress presented a first rising and then declining trend with increase of exogenous Ca2+ concentration.The PPO activity presented a first declining and then rising trend with increase of exogenous Ca2+ concentration.When exogenous Ca2+ concentration was 5 mmol/L, the Pro content and POD activity of two T.kirilowii varieties were 71.3% and 33.0%,and 244.2% and 253.0%,both higher than CK separately.Spraying 5 mmol/L exogenous Ca2+ had different low temperature ameliorating effect of T.kirilowii under low temperature stress,and spraying 5 mmol/L exogenous Ca2+ for 3 d was of the significant low temperature ameliorating effect.