CAJ | 학술논문

为了缩短石斛兰的育种周期，采用正交试验方法，探讨了 ABA、TDZ 及 NAA 对铁皮石斛离体开花的影响，并进行了离体结实及育苗移栽试验。结果表明：TDZ 对铁皮石斛花芽诱导率及正常花开放率的影响分别达极显著水平和显著水平，ABA 和 NAA 则无显著性差异，适宜铁皮石斛离体开花的培养基是 MS＋TDZ（0．05～0．1 mg/L）＋NAA（1．0 mg/L），其花芽诱导率最高达50％，正常花开放率最高达84％；正常结实植株占总授粉植株的30％，与自然栽培植株相比，离体果实发育时间缩短110～140 d，但种子萌发及育苗移栽正常。
위료축단석곡란적육충주기，채용정교시험방법，탐토료 ABA、TDZ 급 NAA 대철피석곡리체개화적영향，병진행료리체결실급육묘이재시험。결과표명：TDZ 대철피석곡화아유도솔급정상화개방솔적영향분별체겁현저수평화현저수평，ABA 화 NAA 칙무현저성차이，괄의철피석곡리체개화적배양기시 MS＋TDZ（0．05～0．1 mg/L）＋NAA（1．0 mg/L），기화아유도솔최고체50％，정상화개방솔최고체84％；정상결실식주점총수분식주적30％，여자연재배식주상비，리체과실발육시간축단110～140 d，단충자맹발급육묘이재정상。
To shorten the breeding cycle of Dendrobium,effects of ABA,TDZ and NAA on in vitro flowering of D.officinale were studied by means of orthogonal test,in vitro seed setting and seedling transplantation were also studied.The results showed that effect of TDZ on the frequency of floral bud induction and normal flowers reached an extremely significant level and a significant level,respectively. While ABA and NAA did not reach significant level.The proper in vitro flowering medium was MS +TDZ (0.05~0.1 mg/ L)+ NAA (1.0 mg/ L),the frequency of floral bud induction was up to 50%, the frequency of normal flowers was up to 84%.Normal seed setting plants accounted for 30% of the total pollination plants,compared with the natural cultivated plants,the development period of in vitro fruit was shortened by 110~140 days,but the seed germination and seedling transplantation were normal.