CAJ | 학술논문

为藜豆的杂交育种及传粉生物学研究等提供基础资料与科学依据，筛选适宜的花粉活力快速测定方法，以白花黑籽藜豆花粉为材料，用光学显微镜观察花粉形态及萌发花粉管的数目，并用 TTC 法、I-KI 法和醋酸洋红法对其花粉活力进行了测定。结果表明：成熟的藜豆花粉近似圆球形，有3个萌发孔，但只有1个萌发孔长出花粉管；TTC 法着色效果差，I-IK 着色时间长（约20 min），而醋酸洋红染色法着色快（约2 min），染色清晰；醋酸洋红法适合藜豆花粉活力的快速测定。
위려두적잡교육충급전분생물학연구등제공기출자료여과학의거，사선괄의적화분활력쾌속측정방법，이백화흑자려두화분위재료，용광학현미경관찰화분형태급맹발화분관적수목，병용 TTC 법、I-KI 법화작산양홍법대기화분활력진행료측정。결과표명：성숙적려두화분근사원구형，유3개맹발공，단지유1개맹발공장출화분관；TTC 법착색효과차，I-IK 착색시간장（약20 min），이작산양홍염색법착색쾌（약2 min），염색청석；작산양홍법괄합려두화분활력적쾌속측정。
In order to provide basic data and scientific evidence for the study of hybrid breeding and pollination biology,and also to select the suitable method for rapid measurement of life viability,the study was carried out to observe the pollen morphology and number of germination pollen tube of S.cochinchinesis by optical microscope,to observe the pollen morphology using TTC staining method,I-KI staining method and aceto-carmine staining method and to measure the life viability of the velvet bean pollen.The results indicated that the mature pollen was of approximately round ball form and had three germination apertures,but only one of which would grow up with pollen tube.The I-KI staining method and the TTC staining were the inappropriate methods for bad staining method and long staining time(about 20 min)respectively.The method of aceto-carmine staining was the best way to determine the pollen viability of S.cochinchinesis for staining rapidly and ideal staining effect.