CAJ | 학술논문

细胞色素P450(CYP450)是动植物及微生物体内一类超基因家族编码的单加氧酶，参与多种催化反应，在生物防御方面具有重要作用。近年来，利用现代生物技术和新一代测序手段及生物信息学分析方法，对参与相关生物代谢合成途径中的CYP450进行了分离、筛选及功能鉴定，进一步阐明了CYP450在植物体内相关代谢途径中的催化机制。本文对植物细胞色素P450（CYP450）基因的分离，在皂苷生物合成、苯丙烷类物质代谢和芥子油苷及IAA生物合成中的功能和表达，以及在人工合成物质的解毒功能及应用等方面的研究做了简要综述。
세포색소P450(CYP450)시동식물급미생물체내일류초기인가족편마적단가양매，삼여다충최화반응，재생물방어방면구유중요작용。근년래，이용현대생물기술화신일대측서수단급생물신식학분석방법，대삼여상관생물대사합성도경중적CYP450진행료분리、사선급공능감정，진일보천명료CYP450재식물체내상관대사도경중적최화궤제。본문대식물세포색소P450（CYP450）기인적분리，재조감생물합성、분병완류물질대사화개자유감급IAA생물합성중적공능화표체，이급재인공합성물질적해독공능급응용등방면적연구주료간요종술。
Cytochrome P450s are a diverse array of mixed-function oxidas in animal,plant and bacteria which consist of a gene superfamily.They play an important role in preventing the plants from injury by catalyzing many kinds of reactions.Several candidate CYP450s that might be involved in ginsenoside biosynthesis have been discovered,screening and functional identification by the modern biotechnology and next generation sequencing technology and bioinformatic analysis in previous studies,further elucidate in the relevant CYP450 catalytic mechanism of metanolic pathways in plants.This article summarized gene isolation,function and expression of ginsenoside biosynthesis pathway briefly, phenylpropanoid metabolic pathway and glucosinolates and IAA biosynthesis pathway,and research in the detoxification function and application of synthetic substances.