CAJ | 학술논문

目的：探讨儿茶酚氧位甲基转移酶（COMT）的基因多态性与利培酮在女性精神分裂症（SCZ）患者中所致的泌乳素升高之间的相关性。方法按照符合美国精神病学会的精神障碍诊断和统计手册第四修订版（DSM-Ⅳ）诊断标准，收集湖北省武汉市武东医院和宁波市康宁医院62例中国汉族女性SCZ患者作为研究对象，对照组选取社区汉族女性健康志愿者，共完成65人。两组民族、性别相同，年龄、文化程度差异无统计学意义。利培酮药物治疗前与治疗8周后分别抽取其外周血液样本，采用微粒酶免疫分析法对用药前后的泌乳素含量进行测定，利用聚合酶链式反应-限制性片段长度多态分析（PCR-RFLP）技术对患者外周血所取提取的基因组DNA进行基因分型。结果 COMT基因Val158/108Met多态性与女性SCZ患者的人口学因素没有明显的关联，入组病例在单药应用利培酮治疗8周后泌乳素水平显著升高（P＜0.001）。而Val/Val基因型患者血液中的泌乳素水平的升高幅度明显地低于其他基因型（P＝0.003）。结论 COMT基因Val158/108Met多态性有望成为预测利培酮治疗女性SCZ所致高泌乳素血症的潜在生物学标记。
목적：탐토인다분양위갑기전이매（COMT）적기인다태성여리배동재녀성정신분렬증（SCZ）환자중소치적비유소승고지간적상관성。방법안조부합미국정신병학회적정신장애진단화통계수책제사수정판（DSM-Ⅳ）진단표준，수집호북성무한시무동의원화저파시강저의원62례중국한족녀성SCZ환자작위연구대상，대조조선취사구한족녀성건강지원자，공완성65인。량조민족、성별상동，년령、문화정도차이무통계학의의。리배동약물치료전여치료8주후분별추취기외주혈액양본，채용미립매면역분석법대용약전후적비유소함량진행측정，이용취합매련식반응-한제성편단장도다태분석（PCR-RFLP）기술대환자외주혈소취제취적기인조DNA진행기인분형。결과 COMT기인Val158/108Met다태성여녀성SCZ환자적인구학인소몰유명현적관련，입조병례재단약응용리배동치료8주후비유소수평현저승고（P＜0.001）。이Val/Val기인형환자혈액중적비유소수평적승고폭도명현지저우기타기인형（P＝0.003）。결론 COMT기인Val158/108Met다태성유망성위예측리배동치료녀성SCZ소치고비유소혈증적잠재생물학표기。
Objective To study the association between COMT polymorphism and the hyperprolactinemia of Risperidone in female schizophrenia patients. Methods A total of 62 Chinese Han schizophrenic patients that examined by trained psychiatrists using the Chinese Classification of Mental Disorders (CCMD-3) criteria were recruited in this retrospective study. A microparticle enzyme immunoassay was used for measuring the serum prolactin levels at the beginning of the study and the time of receiving risperidone treatment on the 8th week. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for COMT genotypes determined. Results There was no significant association between the COMT Val158Met polymorphism and demographic characteristics of the patients in this study. The prolactin level increases significantly after risperidone treatment (P<0.001). However, the Val/Val genotype of the patients showed the lowest prolactin level in all subjects (P=0.003). Conclusion The COMT Val158/108Met polymorphism may be a potential biomarker with predictive value in Risperidone-induced hyperprolactinemia in female schizophrenia patients.