山西中医学院学报
山西中醫學院學報
산서중의학원학보
JOURNAL OF SHANXI COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
1期
29-31
,共3页
更年安片%丹皮酚%五味子醇甲%高效液相色谱法
更年安片%丹皮酚%五味子醇甲%高效液相色譜法
경년안편%단피분%오미자순갑%고효액상색보법
Gengnian'an tablets%paeonol%schizandrin%HPLC
目的：建立同时测定更年安片中丹皮酚和五味子醇甲含量的高效液相色谱法。方法：采用Phenomenex Luna C18色谱柱（4.6 mm×250 mm，5μm），乙腈-0.1%磷酸溶液梯度洗脱，流速1 mL/min，检测波长为260 nm。结果：丹皮酚在0.0818μg~0.491μg范围内、五味子醇甲在0.310μg~1.862μg 范围内与峰面积呈良好线性关系，相关系数分别为1.0000和0.9999，平均回收率分别为98.27%（RSD=0.44%）、101.93%（RSD=1.87%）。结论：该方法简便、准确、重现性好，可作为更年安片质量控制方法之一。
目的：建立同時測定更年安片中丹皮酚和五味子醇甲含量的高效液相色譜法。方法：採用Phenomenex Luna C18色譜柱（4.6 mm×250 mm，5μm），乙腈-0.1%燐痠溶液梯度洗脫，流速1 mL/min，檢測波長為260 nm。結果：丹皮酚在0.0818μg~0.491μg範圍內、五味子醇甲在0.310μg~1.862μg 範圍內與峰麵積呈良好線性關繫，相關繫數分彆為1.0000和0.9999，平均迴收率分彆為98.27%（RSD=0.44%）、101.93%（RSD=1.87%）。結論：該方法簡便、準確、重現性好，可作為更年安片質量控製方法之一。
목적：건립동시측정경년안편중단피분화오미자순갑함량적고효액상색보법。방법：채용Phenomenex Luna C18색보주（4.6 mm×250 mm，5μm），을정-0.1%린산용액제도세탈，류속1 mL/min，검측파장위260 nm。결과：단피분재0.0818μg~0.491μg범위내、오미자순갑재0.310μg~1.862μg 범위내여봉면적정량호선성관계，상관계수분별위1.0000화0.9999，평균회수솔분별위98.27%（RSD=0.44%）、101.93%（RSD=1.87%）。결론：해방법간편、준학、중현성호，가작위경년안편질량공제방법지일。
Objective:To establish a method for content determination of paeonol and schizandrin in Gengnian′an tablets. Method:HPLC was performed on Phenomenex Luna C18 column(4.6 mm×250 mm,5μm)with acetonitrile-0.1%phospho-ric acid solution gradient elution as mobile phase. The flow rate was 1.0 mL/min and detection wavelength was set at 260 nm. Results:The linear relationships of paeonol and schizandrin were respectively good in the range of 0.081 8 μg~0.491μg and 0.310 μg ~1.862 μg with correlation coefficients 1.000 0 and 0.999 9.The average recovery rates were 98.27%(RSD=0.44%)and 101.93%(RSD=1.87%)respectively. Conclusion:This method is convenient,accurate and reliable,and can be used as quality control methods for Gengnian′an tablets.
