CAJ | 학술논문

目的：建立反向高效液相色谱法测定五味子中五味子醇甲、五味子甲素、五味子乙素含量的方法。方法：采用SUNTEK Kromasil C18柱（4.6 mm×250 mm，5μm），Dikma Kromasil C18柱（4.6 mm×250 mm，5μm），以水-甲醇梯度洗脱为流动相，流速：1 mL/min，检测波长：254 nm。结果：五味子醇甲、五味子甲素、五味子乙素进样量均在0.02μg~1.0μg范围内与峰面积有良好线性关系，r2=0.9999、1.0、0.9998；平均回收率分别为98.5%、98.1%、98.6%；RSD分别为1.2%、1.4%、2.4%（n=9）。结论：该方法方便、准确、专属性好，适用于五味子的质量控制。
목적：건립반향고효액상색보법측정오미자중오미자순갑、오미자갑소、오미자을소함량적방법。방법：채용SUNTEK Kromasil C18주（4.6 mm×250 mm，5μm），Dikma Kromasil C18주（4.6 mm×250 mm，5μm），이수-갑순제도세탈위류동상，류속：1 mL/min，검측파장：254 nm。결과：오미자순갑、오미자갑소、오미자을소진양량균재0.02μg~1.0μg범위내여봉면적유량호선성관계，r2=0.9999、1.0、0.9998；평균회수솔분별위98.5%、98.1%、98.6%；RSD분별위1.2%、1.4%、2.4%（n=9）。결론：해방법방편、준학、전속성호，괄용우오미자적질량공제。
Objective:To establish a method for content determination of schizandrin,schizandrin A and schizandrin B in fructus schizandrae by RP-HPLC. Methods:HPLC was performed on SUNTEK Kromasil C18 column (4.6 mm×250 mm,5μm)and Dikma Kromasil C18 column (4.6 mm×250 mm,5μm)with water and MeOH (gradient elution) as mobile phase. Flow rate was 1.0 mL/min and detection wave length was 254 nm. Results:The linear relationship of schizandrin,schizandrin A and schizandrin B was good in the range of 0.02μg~1.0μg with correlation coefficients 0.999 9、1.0、0.999 8. The average recovery rate was 98.5%、98.1%、98.6%and RSD was 1.2%、1.4%、2.4%(n=9). Conclusion:The method is convenient,ac-curate,specific and suitable for the quality control of fructus schizandrae.