CAJ | 학술논문

目的：建立左归丸水提液中总环烯醚萜苷含量测定的方法。方法：水提液通过醇沉后经大孔树脂D101对环烯醚萜苷进行分离纯化，蒸干后加香草醛和高氯酸水浴显色后用紫外可见分光光度计进行含量测定。结果：大孔树脂D101对环烯醚萜苷的分离纯化效果良好，左归丸中环烯醚萜苷以马钱苷含量较高，故选马钱苷为对照品，显色后在24.96μg~99.84μg范围内线性关系良好。结论：该方法可用于左归丸水提液中总环烯醚萜苷的含量测定。
목적：건립좌귀환수제액중총배희미첩감함량측정적방법。방법：수제액통과순침후경대공수지D101대배희미첩감진행분리순화，증간후가향초철화고록산수욕현색후용자외가견분광광도계진행함량측정。결과：대공수지D101대배희미첩감적분리순화효과량호，좌귀환중배희미첩감이마전감함량교고，고선마전감위대조품，현색후재24.96μg~99.84μg범위내선성관계량호。결론：해방법가용우좌귀환수제액중총배희미첩감적함량측정。
Objective:To establish a method for content determination of total iri doid glycosides in water extract from Zuogui pills. Method:Firstly iridoid glycosides was isolated and purified by D101 macroporous resin,then was determined by UV with 534 nm detection wavelength after coloration. Results:D101 macroporous resin eluting with 80% EtOH was suitable for purification of iridoid glycosides.Linear relationship of loganin was good in the range of 24.96 μg ~99.84 μg. Conclusion:This method is suitable for content determination of total iridoid glycosides in water extract from Zuogui pills.