辽宁师范大学学报（自然科学版）
遼寧師範大學學報（自然科學版）
료녕사범대학학보（자연과학판）
JOURNAL OF LIAONING NORMAL UNIVERSITY(NATURAL SCIENCE)
2014年
1期
78-82
,共5页
电化学发光%流动注射分析%酶柱%阿昔洛韦
電化學髮光%流動註射分析%酶柱%阿昔洛韋
전화학발광%류동주사분석%매주%아석락위
electrochemiluminescence%flow-injection analysis%xanthine oxidase%acyclovir
基于阿昔洛韦与酶柱中的黄嘌呤氧化酶反应生成过氧化氢，继而与鲁米诺发生电化学发光反应的原理，建立了流动注射电化学发光测定阿昔洛韦的新方法，并将其应用于实际药品的检测．在pH为9．0的0．05mol/L的硼砂介质、激发电位为0．65V、鲁米诺浓度为5．00×10-4 mol/L、流速为1．90 mL/min的优化条件下，测定阿昔洛韦的线性范围为0．56～22．5mg/L，线性相关系数为0．9995，检出限为0．32mg/L，测定实际药品的相对标准偏差小于1．80％（n＝9），加标回收率为95．5％～108％．
基于阿昔洛韋與酶柱中的黃嘌呤氧化酶反應生成過氧化氫，繼而與魯米諾髮生電化學髮光反應的原理，建立瞭流動註射電化學髮光測定阿昔洛韋的新方法，併將其應用于實際藥品的檢測．在pH為9．0的0．05mol/L的硼砂介質、激髮電位為0．65V、魯米諾濃度為5．00×10-4 mol/L、流速為1．90 mL/min的優化條件下，測定阿昔洛韋的線性範圍為0．56～22．5mg/L，線性相關繫數為0．9995，檢齣限為0．32mg/L，測定實際藥品的相對標準偏差小于1．80％（n＝9），加標迴收率為95．5％～108％．
기우아석락위여매주중적황표령양화매반응생성과양화경，계이여로미낙발생전화학발광반응적원리，건립료류동주사전화학발광측정아석락위적신방법，병장기응용우실제약품적검측．재pH위9．0적0．05mol/L적붕사개질、격발전위위0．65V、로미낙농도위5．00×10-4 mol/L、류속위1．90 mL/min적우화조건하，측정아석락위적선성범위위0．56～22．5mg/L，선성상관계수위0．9995，검출한위0．32mg/L，측정실제약품적상대표준편차소우1．80％（n＝9），가표회수솔위95．5％～108％．
We report a new flow-injection electrochemiluminescence (ECL ) analytical method for the determination of aciclovir based on the enzymatic reaction of aciclovir by xanthine oxidase immobi-lized in a micro-column .When the sample flow s through the enzyme reactor ,the xanthine oxidase cat-alyzes aciclovir to produce hydrogen peroxide (H2 O2 ) .Followed by ECL reaction of H2 O2 with lumi-nol ,the acyclovir was determined in the sample .In the paper ,effects of the mobile phase ,electrolyte pH ,excitation potential ,and luminol concentration on the ECL intensity were investigated and opti-mized .The linear response of aciclovir concentrations ranged from 0 .56 to 22 .5 mg/L was obtained with a detection limit of 0 .32 mg/L .This method was successfully applied to detect the active ingre-dient of acyclovir in pharmaceutical preparations .The relative standard deviation was less than 1 .80% (n=9) and the recovery was in the range of 95 .5% ~108% for the determination of actual samples .
