CAJ | 학술논문

目的：用补中益气汤含药血清处理A549/DDP细胞，观测药物泵P-gp及LRP的表达，并同小干扰RNA片段（siRNA）靶向切除P-gp、LRP基因后对这两种蛋白表达的影响相比较．方法：补中益气汤含药血清处理A549/DDP细胞，设计并合成针对P-gp、LRP基因对应序列的siRNA，采用转染试剂对细胞进行转染．两种蛋白实验分组均为：空白对照组、含药血清处理组及siRNA处理组．采用RT-PCR检测P-gp及LRP mRNA，免疫细胞化学法检测蛋白表达及定位，WB检测蛋白表达．结果：补中益气汤含药血清可以降低两种药物泵的表达，效果同siRNA类似，具有统计学差异（P＜0.05）．结论：补中益气汤含药血清能降低A549/DDP细胞上P-gp和LRP的表达．
목적：용보중익기탕함약혈청처리A549/DDP세포，관측약물빙P-gp급LRP적표체，병동소간우RNA편단（siRNA）파향절제P-gp、LRP기인후대저량충단백표체적영향상비교．방법：보중익기탕함약혈청처리A549/DDP세포，설계병합성침대P-gp、LRP기인대응서렬적siRNA，채용전염시제대세포진행전염．량충단백실험분조균위：공백대조조、함약혈청처리조급siRNA처리조．채용RT-PCR검측P-gp급LRP mRNA，면역세포화학법검측단백표체급정위，WB검측단백표체．결과：보중익기탕함약혈청가이강저량충약물빙적표체，효과동siRNA유사，구유통계학차이（P＜0.05）．결론：보중익기탕함약혈청능강저A549/DDP세포상P-gp화LRP적표체．
Aim:To discuss the effect of Center-Supplementing and Qi-Boosting Decoction (补中益气汤,CQD)serum on the expressing of drug pumps P-glycoprotein (P-gp)and lung resistance protein (LRP)in A549/DDP cells,and compared the results to the expressing of the two drug pumps in A549/DDP cells that affected by small interfering RNA.Methods:The cell is cultured with CQD serum,and the siRNAs which had the same sequences of P-gp and LRP gene were designed and synthesized,then the human lung adenocarcinoma drug-resistant cell-line A549/DPP transected with Transfection Reagent. Both of the experimental cells were grouped into control group,CQD serum group and siRNA-processed group.Reverse transcription-polymerase chain reaction (RT-PCR)was applied to determine the mRNA expressions of P-gp and LRP mRNA,Western blotting and immunocytochemical method was adopted to determine the protein expression of P-gp and LRP.Results:CQD serum can decrease the expression of both of the drug pumps and the function is similar to that of siRNA.Conclusion:CQD serum serves ef-fectively in decrease the protein expression of P-gp and LRP.