CAJ | 학술논문

目的：探讨葛根素对氧化应激状态下人视网膜色素上皮细胞（ ARPE-19 cells ）的保护作用。方法用200μmol/L过氧化氢（ H2 O2）诱导氧化应激，不同浓度葛根素作用于ARPE-19细胞24 h。应用比色法观察每组中超氧化物歧化酶（ SOD）、谷胱甘肽（ GSH）及丙二醛（ MDA）含量的变化。结果H2 O2能够降低SOD活性，降低GSH含量，并且增加MDA含量，应用葛根素剂量依赖性地减少了以上效应。结论葛根素能够通过调节细胞内抗氧化标记物，保护人视网膜色素上皮细胞抵御H2 O2诱导的氧化应激。
목적：탐토갈근소대양화응격상태하인시망막색소상피세포（ ARPE-19 cells ）적보호작용。방법용200μmol/L과양화경（ H2 O2）유도양화응격，불동농도갈근소작용우ARPE-19세포24 h。응용비색법관찰매조중초양화물기화매（ SOD）、곡광감태（ GSH）급병이철（ MDA）함량적변화。결과H2 O2능구강저SOD활성，강저GSH함량，병차증가MDA함량，응용갈근소제량의뢰성지감소료이상효응。결론갈근소능구통과조절세포내항양화표기물，보호인시망막색소상피세포저어H2 O2유도적양화응격。
Objective To investigate the protective effect of puerarin on ARPE-19 cells against oxidative stress.Methods ARPE-19 cells exposed to 200μmol/L hydrogen peroxide (H2O2) were incubated with various concentrations of puerarin for 24 hours.The activity of the superox-ide dismutase ( SOD) , and the change of the content of glutathion ( GSH) and malonaldehyde ( MDA) in each group were observed by chromatometry .Results H2 O2 decreased the activity of SOD, decreased the content of GSH and increased the content of MDA , that were reduced with different concentrations of puerarin in a dose-dependent manner .Conclusion Puerarin could protect ARPE-19 cells against H 2 O2-induced oxidative stress through modulation of intra-cellular antioxidant biomarkers .