CAJ | 학술논문

为了解新疆阿勒泰地区的捕食线虫性真菌分布情况,采用玉米粉琼脂培养基( CMA)培养分离阿勒泰地区土壤中的捕食线虫性真菌,通过对纯化后菌株的形态学观察和分子生物学分析,鉴定出5株少孢节丛孢属真菌和1株圆锥节丛孢菌。另外用质量浓度分别为0.5,0.05,0.005 g/L的L-苯丙氨酸、L-色氨酸、L-蛋氨酸和L-缬氨酸对已分离的圆锥节丛孢菌AS3株进行捕食器生成的诱导反应,发现各组氨基酸对捕食器的产生均有诱导效应,但质量浓度为0.05 g/L的L-苯丙氨酸诱导产生的捕食器总数最多。为利用捕食线虫性真菌进行生物防控奠定了理论基础。
위료해신강아륵태지구적포식선충성진균분포정황,채용옥미분경지배양기( CMA)배양분리아륵태지구토양중적포식선충성진균,통과대순화후균주적형태학관찰화분자생물학분석,감정출5주소포절총포속진균화1주원추절총포균。령외용질량농도분별위0.5,0.05,0.005 g/L적L-분병안산、L-색안산、L-단안산화L-힐안산대이분리적원추절총포균AS3주진행포식기생성적유도반응,발현각조안기산대포식기적산생균유유도효응,단질량농도위0.05 g/L적L-분병안산유도산생적포식기총수최다。위이용포식선충성진균진행생물방공전정료이론기출。
Nematode-trapping fungi were isolated with corn meal agar( CMA) from soils samples of Altay region in Xinjiang Province. 6 strains were successfully isolated and five strains identified as Arthrobotrys oligospora and the other one is Arthrobotrys conoides through morphological characteristics and molecular biology analysis. In addition, four amino acids (L-phenylalanine,L-tryptophan,L-methionine and L-valine) in different concentrations (0. 5, 0. 05,0. 005 g/L) were used as revulsive to induce the nematode-trapping organs forming of AS3 strain. The results showed that all the amino acids had effects on the traps forming,but the L-phenylalanine in concentration of 0. 05 g/L induced maximum number of trapping devices. This study laid a good foundation for biological prevention and control by nematode-trapping fungi.