CAJ | 학술논문

采用系列稀释法和平板涂布法从黑龙江漠河地区的永久冻土层20份土壤样品中分离得到172株细菌菌株，经平板对峙法初筛得到10株拮抗菌，经复筛得到了1株对甘蓝枯萎病菌有较强抑制作用的拮抗细菌MH71菌株。通过菌落形态观察、生理生化测定、16S rDNA和16S~23S rDNA之间的ITS特异性序列分析等，表明MH71菌株为解淀粉芽孢杆菌( Bacillus amyloliquefaciens)。该菌株对镰刀病菌、灰葡萄孢及炭疽病菌等15种农业生产上常见的植物病原真菌和黄瓜角斑、平菇褐斑、大白菜黑腐和啤酒酵母菌等植物病原细菌和酵母菌均有不同程度的拮抗作用，表现出广谱抗菌活性。此结果表明：MH71菌株是一株极具开发潜力的生防菌株。
채용계렬희석법화평판도포법종흑룡강막하지구적영구동토층20빈토양양품중분리득도172주세균균주，경평판대치법초사득도10주길항균，경복사득도료1주대감람고위병균유교강억제작용적길항세균MH71균주。통과균락형태관찰、생리생화측정、16S rDNA화16S~23S rDNA지간적ITS특이성서렬분석등，표명MH71균주위해정분아포간균( Bacillus amyloliquefaciens)。해균주대렴도병균、회포도포급탄저병균등15충농업생산상상견적식물병원진균화황과각반、평고갈반、대백채흑부화비주효모균등식물병원세균화효모균균유불동정도적길항작용，표현출엄보항균활성。차결과표명：MH71균주시일주겁구개발잠력적생방균주。
172 strains of bacteria were isolated from 20 soil samples collected from the permafrost of Mohe are-a,Heilongjiang Province, by the serial dilution and spread-plate methods. Plate stand-opposite culture method for preliminary screening of 10 antagonistic strains and one bacterial strain MH71,exhibited an extensive antagonistic effect on Fusarium oxysporum f. sp. conglutinans. The strain was identified as Bacillus amyloliquefaciens based on morphological observation, physiological and biochemical characteristics, 16S rDNA sequence homology and 16S-23S ITS specific PCR detection. Moreover,the strain showed broad-spectrum antimicrobial activities,such as Fusarium spp. ,Botrytis cinerea,Colletotrichum sp. ,15 plant pathogenic fungi and Pseudomonas syringae pv. lachry-mans,P. tollasii and Xanthomonas campestris pv. campsetris plant pathogenic bacteria and Saccharomyces cerevisiae. Results showed that strain MH71 had a great potential on bio-control application.