CAJ | 학술논문

旨为研究鹅ENO1基因分子特征,阐明不同繁殖期鹅下丘脑、垂体和卵巢组织ENO1表达规律。应用生物信息学技术分析并预测四川白鹅ENO1结构和功能,实时荧光定量PCR技术检测产蛋前期(6月龄)和产蛋期(8月龄)鹅下丘脑、垂体和卵巢组织ENO1的表达量。结果表明,四川白鹅ENO1是内源性蛋白质,含3个N-糖基化位点和16个磷酸化位点,二级结构以α-螺旋和无规则卷曲为主,主要分布于细胞质(73.9%)和细胞核(13.0%)。四川白鹅ENO1的4个Mg2+结合位点和1个烯醇化酶标签进化保守,但是其纤溶酶原受体结构域和MBP-1结构域分别与人氨基酸序列存在4处(422Arg→Lys、432Arg→Leu、433Ile→Ala和434Asn→Lys)和5处变异(124Ala→Val、141Pro→Ser、176Asp→Ala、177Thr→Asn和223Ala→Gly)。产蛋期鹅下丘脑、垂体和卵巢组织ENO1表达量分别是产蛋前期的1.80(P<0.05),1.64(P<0.05)和3.05倍(P<0.05)。四川白鹅ENO1是多功能的、进化保守的内源性蛋白质,ENO1可通过调节HPG轴功能来参与调控鹅的繁殖功能。
지위연구아ENO1기인분자특정,천명불동번식기아하구뇌、수체화란소조직ENO1표체규률。응용생물신식학기술분석병예측사천백아ENO1결구화공능,실시형광정량PCR기술검측산단전기(6월령)화산단기(8월령)아하구뇌、수체화란소조직ENO1적표체량。결과표명,사천백아ENO1시내원성단백질,함3개N-당기화위점화16개린산화위점,이급결구이α-라선화무규칙권곡위주,주요분포우세포질(73.9%)화세포핵(13.0%)。사천백아ENO1적4개Mg2+결합위점화1개희순화매표첨진화보수,단시기섬용매원수체결구역화MBP-1결구역분별여인안기산서렬존재4처(422Arg→Lys、432Arg→Leu、433Ile→Ala화434Asn→Lys)화5처변이(124Ala→Val、141Pro→Ser、176Asp→Ala、177Thr→Asn화223Ala→Gly)。산단기아하구뇌、수체화란소조직ENO1표체량분별시산단전기적1.80(P<0.05),1.64(P<0.05)화3.05배(P<0.05)。사천백아ENO1시다공능적、진화보수적내원성단백질,ENO1가통과조절HPG축공능래삼여조공아적번식공능。
The objective of this work was to elucidate the molecular characteristic and expression profiling of ENO1 in hypothalamic,pituitary and ovarian tissues of geese during different reproductive stages. The structure and function of ENO1 was analyzed by bioinformatics tools. The level of ENO1 gene expression in hypothalamus,pituita-ry gland and ovary was demonstrated by quantitative real-time PCR in Sichuan white geese during prelaying ( at 6 months of age) and laying( at 8 months of age) stages. ENO1 of Sichuan white goose was an endogenous protein. It was predicted that 3 N-glycosylation sites and 16 phosphorylation sites might exist in the amino acid sequence of goose ENO1 .α-helix and extended strand was predominant secondary structure of goose ENO1 . ENO1 was predicted to be primarily localized in the cytoplasm(73. 9%) and nucleus(13. 0%). ENO1 included conserved 4 Mg2+ bind-ing sites and one enolase signature. Compared with ENO1 amino acid sequence in Homo Sapiens,there were four changes of amino acid residues in plasminogen receptor domain(422Arg→Lys,432Arg→Leu,433Ile→Ala and 434Asn→Lys) and five changes of amino acids occurred in the functional domain of MBP-1(124Ala→Val,141Pro→Ser,176Asp→Ala,177Thr→Asn and 223Ala→Gly) in goose ENO1 amino acid sequence. ENO1 expression of the hypothalamus,pituitary gland and ovary in laying geese was 1. 80,1. 64 and 3. 05(P<0. 05) folds compared with prelaying geese,respectively. These results suggest that Sichuan white goose ENO1 is a conserved,multi-func-tionally endogenous protein. ENO1 may regulate the process of goose reproduction via mediating executive function of HPG axis.