CAJ | 학술논문

为明确10个枸杞品种在DNA分子水平上的遗传差异,利用ISSR分子标记技术对其多态性进行了分析。试验从100个ISSR引物中筛选出8个适宜引物,8个引物共扩增出90条清晰可辨的条带,其中44条带具有多态性,多态性条带百分率为48.9%,通过聚类分析,以阈值0.41为基准,把供试的10个材料划分为2个类群,宁杞5号和张成枸杞的遗传距离为0.6296,遗传关系最远。这将为枸杞的种质遗传多样性分析和杂交育种提供了初步理论依据。
위명학10개구기품충재DNA분자수평상적유전차이,이용ISSR분자표기기술대기다태성진행료분석。시험종100개ISSR인물중사선출8개괄의인물,8개인물공확증출90조청석가변적조대,기중44조대구유다태성,다태성조대백분솔위48.9%,통과취류분석,이역치0.41위기준,파공시적10개재료화분위2개류군,저기5호화장성구기적유전거리위0.6296,유전관계최원。저장위구기적충질유전다양성분석화잡교육충제공료초보이론의거。
To know the genetic differences in DNA level among 10 Chinese wolfberry varieties,the polymorphism among the tested plants were analyzed based on ISSR molecular marker. From 100 ISSR molecular primers,eight were selected and used for genetic diversity analyisis for the 10 Chinese wolfberry accessions. Ninety clear bands were am-plified,in which 44 were polymorphic,accounting for 48. 9%. At genetic distance 0. 41,the 10 Chinese wolfberry ac-cessions could be clustered into 2 groups. Ningqi 5 and Gouqi zhangcheng had the farthest genetic distance (0. 629 6). The result provided the theoretical basis for Chinese wolfberry genetic diversity and hybridization breeding.