华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
1期
78-82
,共5页
李芦江%陈文生%兰海%潘光堂%杨克诚
李蘆江%陳文生%蘭海%潘光堂%楊剋誠
리호강%진문생%란해%반광당%양극성
双亲混合选择%遗传多样化%玉米%窄基群体%SSR
雙親混閤選擇%遺傳多樣化%玉米%窄基群體%SSR
쌍친혼합선택%유전다양화%옥미%착기군체%SSR
Biparental mass selection ( MS)%Genetic diversity%Maize%Population%SSR
以玉米群体P4C0及其经过5轮控制双亲混合选择的改良群体为材料,利用SSR分子标记分析其遗传多样性变化。结果表明,经2~3轮选择后,从总体上看,群体多态位点数、多态位点比例、群体内单株间遗传距离、基因杂合度和遗传多样性指数等遗传多样性参数并未发生明显的变化,而进行5轮改良后,虽然每轮减小幅度较小,但累计减少较多。群体基因型数总体上是减小的趋势,但不同地点改良群体基因型数表现减少与增加交替出现的现象。以上结果表明,在改良低代,群体遗传多样性得到了较好的保持,而多代改良则可能会导致群体遗传多样性的下降;在不同生态条件下进行穿梭式群体改良,对加快改良进程,保持群体遗传多样性和提高选择效率都有一定的作用。
以玉米群體P4C0及其經過5輪控製雙親混閤選擇的改良群體為材料,利用SSR分子標記分析其遺傳多樣性變化。結果錶明,經2~3輪選擇後,從總體上看,群體多態位點數、多態位點比例、群體內單株間遺傳距離、基因雜閤度和遺傳多樣性指數等遺傳多樣性參數併未髮生明顯的變化,而進行5輪改良後,雖然每輪減小幅度較小,但纍計減少較多。群體基因型數總體上是減小的趨勢,但不同地點改良群體基因型數錶現減少與增加交替齣現的現象。以上結果錶明,在改良低代,群體遺傳多樣性得到瞭較好的保持,而多代改良則可能會導緻群體遺傳多樣性的下降;在不同生態條件下進行穿梭式群體改良,對加快改良進程,保持群體遺傳多樣性和提高選擇效率都有一定的作用。
이옥미군체P4C0급기경과5륜공제쌍친혼합선택적개량군체위재료,이용SSR분자표기분석기유전다양성변화。결과표명,경2~3륜선택후,종총체상간,군체다태위점수、다태위점비례、군체내단주간유전거리、기인잡합도화유전다양성지수등유전다양성삼수병미발생명현적변화,이진행5륜개량후,수연매륜감소폭도교소,단루계감소교다。군체기인형수총체상시감소적추세,단불동지점개량군체기인형수표현감소여증가교체출현적현상。이상결과표명,재개량저대,군체유전다양성득도료교호적보지,이다대개량칙가능회도치군체유전다양성적하강;재불동생태조건하진행천사식군체개량,대가쾌개량진정,보지군체유전다양성화제고선택효솔도유일정적작용。
Five cycles of biparental mass selection ( MS ) were carried out in a narrow base synthetic maize population namely P4C0. The genetic diversity of 6 maize populations,including the basic population and the devel-oped populations,were evaluated by 51 SSR markers. The results showed that the number of polymorphic loci,the percentage of polymorphic loci,the mean genetic distance,the expected heterozygosity ( He) and the Shannon ge-netic diversity index ( I) displayed a descending trend with the advance of selection,while the change after 2 to 3 cycles were not significant. After 5 cycles,the cumulative reduction is larger although the decrease were slight for per cycle. The number of genotypes selected also decreases with selection presented the trend of declining with the advance of selection and related to the location where the populations were developed. It concluded that the genetic diversity maintained in early generations,especially in the 1st and 2nd cycles,while it declined in advance cycles of MS. The alternative improvement of population in different ecological environments was advantageous to the selection of favorable genes.