CAJ | 학술논문

以马铃薯X病毒载体PVX 为工具,探究不同马铃薯品种和不同侵染方式对PVX介导的VIGS沉默体系的影响,结果表明：马铃薯基因型的差异是影响VIGS沉默效果的主要因素。通过对VIGS沉默pds(八氢茄红素脱氢酶)基因的植株进行半定量RT-PCR检测,发现在所有供试的马铃薯品种中,乌盟601中pds基因的沉默效果最好,侵染后pds基因表达量下降了79.8%；而虎头、夏坡蒂和大西洋中的pds基因表达量也有不同程度降低,分别为64.1%,31．0%,4.5%。不同侵染方式即叶片注射和茎秆刺伤的试验结果表明,VIGS沉默效果和侵染方式的相关性不显著,采用叶片注射和茎秆刺伤接种马铃薯品种乌盟601,pds基因表达量分别下降了59.0%和56.5%；而虎头叶片注射和茎秆刺伤接种pds基因表达量分别下降了34.7%和40.3%。方差分析结果表明,2种不同的接种方法对pds基因沉默比例没有显著差异。
이마령서X병독재체PVX 위공구,탐구불동마령서품충화불동침염방식대PVX개도적VIGS침묵체계적영향,결과표명：마령서기인형적차이시영향VIGS침묵효과적주요인소。통과대VIGS침묵pds(팔경가홍소탈경매)기인적식주진행반정량RT-PCR검측,발현재소유공시적마령서품충중,오맹601중pds기인적침묵효과최호,침염후pds기인표체량하강료79.8%；이호두、하파체화대서양중적pds기인표체량야유불동정도강저,분별위64.1%,31．0%,4.5%。불동침염방식즉협편주사화경간자상적시험결과표명,VIGS침묵효과화침염방식적상관성불현저,채용협편주사화경간자상접충마령서품충오맹601,pds기인표체량분별하강료59.0%화56.5%；이호두협편주사화경간자상접충pds기인표체량분별하강료34.7%화40.3%。방차분석결과표명,2충불동적접충방법대pds기인침묵비례몰유현저차이。
In this study,factors which affect the silence system of VIGS in potato was unravel,the experiment results suggested that the genotype is the main factor to affect the result of VIGS. Among all the tested potato varie-ties,VIGS showed the highest silence effect in potato variety Wumeng 601,the pds gene expression level was de-cressed by 79. 8%;and the pds gene expression level in Hutou,Shepody and Atlantic was decreased by 64. 1%, 31. 0% and 4. 5% respectively. The effects of inoculation ways such as leaf injection and stem scrub on the silence result of VIGS was also tested,the results suggested that the correlation between inoculation ways and silence effect was not very strong. In Wumeng 601,the pds gene expression level of leaf injection and stem scrub were decreased by 59. 0% and 56. 5% separately;it is 34. 7% and 40. 3% respectively in potato variety Hutou. The analysis of va-riance ( ANOVA) showed there does not have significant difference between the two inoculation ways.