CAJ | 학술논문

小麦TaNADP-ME1基因对干旱、盐、低温等非生物胁迫作出响应。利用重组技术成功构建了TaNADP-ME1的植物表达载体pCAME1,农杆菌介导法成功转化水稻品种日本晴成熟胚诱导的愈伤组织,通过潮霉素筛选获得了抗性愈伤,分化和生根后获得转化植株,PCR鉴定获得20株转基因水稻苗。结果为进一步确定TaNADP-ME1的基因功能奠定了基础。
소맥TaNADP-ME1기인대간한、염、저온등비생물협박작출향응。이용중조기술성공구건료TaNADP-ME1적식물표체재체pCAME1,농간균개도법성공전화수도품충일본청성숙배유도적유상조직,통과조매소사선획득료항성유상,분화화생근후획득전화식주,PCR감정획득20주전기인수도묘。결과위진일보학정TaNADP-ME1적기인공능전정료기출。
The wheat TaNADP-ME1 gene was responsive to drought,salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology,transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method,acquired resistant callus by hy-gromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were ac-quired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.