CAJ | 학술논문

蚜虫作为病毒的传播介体为害植物造成的经济损失远远超过蚜虫本身,因此急需建立快速、准确、灵敏的蚜虫带毒检测方法。根据百合无症病毒( LSV)和烟草花叶病毒( TMV)外壳蛋白基因保守序列设计引物与探针,制备基因芯片；用 Cy3标记下游引物,提取饲毒( LSV和TMV)棉蚜、桃蚜总RNA,RT-PCR扩增产物与芯片杂交,荧光扫描仪检测并分析信号。结果表明,该芯片可以同时检测上述2种蚜虫的带毒情况,此方法有较好的特异性和重复性,能快速、准确地对介体蚜虫的带毒情况进行检测。
아충작위병독적전파개체위해식물조성적경제손실원원초과아충본신,인차급수건립쾌속、준학、령민적아충대독검측방법。근거백합무증병독( LSV)화연초화협병독( TMV)외각단백기인보수서렬설계인물여탐침,제비기인심편；용 Cy3표기하유인물,제취사독( LSV화TMV)면아、도아총RNA,RT-PCR확증산물여심편잡교,형광소묘의검측병분석신호。결과표명,해심편가이동시검측상술2충아충적대독정황,차방법유교호적특이성화중복성,능쾌속、준학지대개체아충적대독정황진행검측。
Aphid transmission of the virus as a mediator harm plants far more than economic losses caused by aphids themselves. So,rapid,sensitive and specific method,by which viruses carried by aphids can be detected sim-ultaneously,is needed urgently. In this study,according to the Lily symptomless virus ( LSV) and the Tobacco mosa-ic virus ( TMV) coat protein gene sequence,primers and probes were designed,gene chip was prepared. The down-stream primers were labeled by Cy3 . The total RNA was extracted from Aphis gossypii and Myzus perdicae raised with lily infected by LSV and TMV respectively. The product amplified by RT-PCR using the labeled primers was hybrid-ized with the gene chip. The hybridization signal was detected and analyzed by a fluorescence scanner. The result showed that the chip can simultaneously detect both aphids infected cases,this method has good specificity and re-producibility,fast and accurate on the mediator infected aphids were detected.