CAJ | 학술논문

以苗期杂交水稻威优916为试验材料,分别以蒸馏水和8 mg/L 6-苄基腺嘌呤溶液培养,通过对功能叶片蛋白质组差异表达分析,以揭示养分胁迫下6-苄基腺嘌呤延缓水稻衰亡的效应及其机理。叶片全蛋白质经双向电泳分离,获得了22个响应6-苄基腺嘌呤处理的蛋白质,经质谱分析,其中15个功能得到鉴定,涉及光合作用、呼吸作用、激素代谢及逆境反应等多个代谢途径。分析结果显示,养分胁迫下,6-BA诱导了叶片中光合蛋白质、呼吸代谢相关蛋白质的表达量上调,减少衰亡发生密切相关蛋白质的积累,从而提高叶片光合能力,促进呼吸,满足植株生长对物质及能量的需求,延缓植株衰亡。
이묘기잡교수도위우916위시험재료,분별이증류수화8 mg/L 6-변기선표령용액배양,통과대공능협편단백질조차이표체분석,이게시양분협박하6-변기선표령연완수도쇠망적효응급기궤리。협편전단백질경쌍향전영분리,획득료22개향응6-변기선표령처리적단백질,경질보분석,기중15개공능득도감정,섭급광합작용、호흡작용、격소대사급역경반응등다개대사도경。분석결과현시,양분협박하,6-BA유도료협편중광합단백질、호흡대사상관단백질적표체량상조,감소쇠망발생밀절상관단백질적적루,종이제고협편광합능력,촉진호흡,만족식주생장대물질급능량적수구,연완식주쇠망。
In order to reveal the mechanism of 6-benzyl adenine deferring contabescence of rice,the hybrid rice Weiyou 916 seedlings were cultivated with distilled water and 6-benzyl adenine(8 mg/L) ,respectively,and the pro-teins expression profile of functional leaves were investigated by using the approach of proteomics. Proteins were ex-tracted from rice functional leaf and separated by two-dimensional gel electrophoresis,then 22 differential expression proteins that induced by 6-benzyl adenine were obtained,among which 15 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, and categorized into 4 groups of functions including photosynthesis,respiration,hormone metabolism and stress response. Results showed that under nutrient stress con-dition,6-benzyl adenine had benifical effect on rice plant:the expression abundance of proteins associated with pho-tosynthesis and respiration increased,and proteins associated with contabescence were down-regulated in expression abundance. Thereby,application of 6-benzyl adenine chould enhanced the photosynthesis and respiration directly, provided the material and energy for plant growth and defered rice contabescence.