CAJ | 학술논문

以口岸经常截获的红脂大小蠹、黄杉大小蠹、山松大小蠹、间大小蠹、红翅大小蠹和落叶松大小蠹等6种大小蠹属昆虫为样本，根据其昆虫检索表制作了近缘关系树；利用分子手段对其线粒体COI基因进行扩增，分析同源序列的多样性、遗传距离和系统进化关系，并将近缘关系树和分子进化树进行比较，研究利用分子手段对大小蠹属昆虫进行快速鉴定的可行性和准确性。结果表明：同种的大小蠹碱基差异较小，而不同种间的碱基差异较大，能明显区分出不同种；大小蠹属的遗传距离和邻接法系统进化树也证实，分子鉴定结果和检索表分类的结果一致。可利用分子鉴定的方法对不同种的大小蠹属昆虫进行快速准确的鉴定。
이구안경상절획적홍지대소두、황삼대소두、산송대소두、간대소두、홍시대소두화락협송대소두등6충대소두속곤충위양본，근거기곤충검색표제작료근연관계수；이용분자수단대기선립체COI기인진행확증，분석동원서렬적다양성、유전거리화계통진화관계，병장근연관계수화분자진화수진행비교，연구이용분자수단대대소두속곤충진행쾌속감정적가행성화준학성。결과표명：동충적대소두감기차이교소，이불동충간적감기차이교대，능명현구분출불동충；대소두속적유전거리화린접법계통진화수야증실，분자감정결과화검색표분류적결과일치。가이용분자감정적방법대불동충적대소두속곤충진행쾌속준학적감정。
Taking Dendrotonus valens,D.pseudotsug,D.ponderosae,D.adjunctus,D.rufipennis and D.simplex 6 Dendrotonus species often intercepted in ports as the smaples,this paper constructed a Close Sib Relationship Tree;it used molecule measures to amplify mitochondrial COI genes,it analysed the relationships among same source sequence diversity,heredity distance and system evolution, and Close Sib Relationship Tree and Molecule Evolution Tree were compared,and it researched the feasibility and accuracy of Dend-rotonus insects rapid identification with the molecular techniques.The results showed that the homogeneous Dendrotonus bases had the small difference and different kinds of it had the larger difference,the different species could be clearly distinguished;the Dendro-tonus genetic distance and Neighbour-Joining System Evolution Tree also confirmed that the molecular identification results and the retrieval table classification results were the same.The molecular identification methods were used to do the fast accurate identifica-tion for different kinds of Dendrotonus insects.