浙江大学学报(农业与生命科学版)
浙江大學學報(農業與生命科學版)
절강대학학보(농업여생명과학판)
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2014年
2期
165-174
,共10页
朱波%曾建斌%吴德志%蔡圣冠%杨丽娜%张国平
硃波%曾建斌%吳德誌%蔡聖冠%楊麗娜%張國平
주파%증건빈%오덕지%채골관%양려나%장국평
青藏高原%野生大麦%基因型%耐低钾%生理特性
青藏高原%野生大麥%基因型%耐低鉀%生理特性
청장고원%야생대맥%기인형%내저갑%생리특성
Tibetan Plateau%wild barley%genotype%low potassium tolerance%physiological trait
以99份青藏高原一年生野生大麦基因型为材料,通过水培试验比较它们对低钾耐性的基因型差异。结果显示,供试的野生大麦基因型在低钾耐性上差异显著,其中,XZ107、XZ153和 XZ159表现出较高的低钾耐性,而XZ141表现为低钾敏感。然后以上述4种低钾耐性不同的野生大麦基因型和钾素利用效率不同的2个栽培品种为材料,研究它们在低钾胁迫条件下的钾素利用效率、净光合速率和 HvHAK1表达量的差异。结果表明,以上参数值均为耐低钾基因型显著高于低钾敏感基因型。另外,利用 DArT 标记进行遗传关联分析表明,大麦组织中的钾含量受多基因控制。表明青藏高原一年生野生大麦可为栽培大麦及其他作物的钾素高效育种提供有益的种质及基因,在低钾与正常钾素水平下的相对干物质质量和钾素利用效率是评估大麦低钾耐性的可靠指标。
以99份青藏高原一年生野生大麥基因型為材料,通過水培試驗比較它們對低鉀耐性的基因型差異。結果顯示,供試的野生大麥基因型在低鉀耐性上差異顯著,其中,XZ107、XZ153和 XZ159錶現齣較高的低鉀耐性,而XZ141錶現為低鉀敏感。然後以上述4種低鉀耐性不同的野生大麥基因型和鉀素利用效率不同的2箇栽培品種為材料,研究它們在低鉀脅迫條件下的鉀素利用效率、淨光閤速率和 HvHAK1錶達量的差異。結果錶明,以上參數值均為耐低鉀基因型顯著高于低鉀敏感基因型。另外,利用 DArT 標記進行遺傳關聯分析錶明,大麥組織中的鉀含量受多基因控製。錶明青藏高原一年生野生大麥可為栽培大麥及其他作物的鉀素高效育種提供有益的種質及基因,在低鉀與正常鉀素水平下的相對榦物質質量和鉀素利用效率是評估大麥低鉀耐性的可靠指標。
이99빈청장고원일년생야생대맥기인형위재료,통과수배시험비교타문대저갑내성적기인형차이。결과현시,공시적야생대맥기인형재저갑내성상차이현저,기중,XZ107、XZ153화 XZ159표현출교고적저갑내성,이XZ141표현위저갑민감。연후이상술4충저갑내성불동적야생대맥기인형화갑소이용효솔불동적2개재배품충위재료,연구타문재저갑협박조건하적갑소이용효솔、정광합속솔화 HvHAK1표체량적차이。결과표명,이상삼수치균위내저갑기인형현저고우저갑민감기인형。령외,이용 DArT 표기진행유전관련분석표명,대맥조직중적갑함량수다기인공제。표명청장고원일년생야생대맥가위재배대맥급기타작물적갑소고효육충제공유익적충질급기인,재저갑여정상갑소수평하적상대간물질질량화갑소이용효솔시평고대맥저갑내성적가고지표。
Summary First,a hydroponic experiment was conducted to compare the difference in low potassium (K) tolerance among 99 Tibetan annual wild barley genotypes.The wild barleys that were examined showed a significant difference in low K tolerance,with XZ107,XZ1 53 and XZ1 59 being high tolerant,and XZ141 being high sensitive.Second,these four wild barley genotypes as well as two cultivated barley cultivars were used to investigate the genotypic difference in K use efficiency(KUE),net photosynthesis and expression of HvHAK1 . The results showed that the values of these parameters were larger in low K tolerant genotypes than in sensitive ones.In addition, the association analysis using DArT markers indicated that tissue K concentration was controlled by multiple genes.The current results also showed that Tibetan annual wild barley may provide elite germplasm or genes for genetic improvement of KUE in barley and other crops,and relative tissue biomass as well as KUE under low K relative to normal K level can be used as the reliable index for evaluating low K tolerance in barley.