CAJ | 학술논문

以82份西藏野生大麦和16份栽培大麦为材料，利用水培试验，以生物量和氮素吸收为指标，比较了它们耐低氮的基因型差异。结果表明低氮耐性的基因型差异显著，野生大麦的变异大于栽培大麦。在此基础上，以鉴定获得的2个耐低氮野生大麦（XZ113和 XZ149），1个中度耐低氮野生大麦（XZ125）和1个耐低氮栽培大麦品种（ZD9）为材料，研究它们在不同氮浓度下的生长与生理表现。氮素吸收动力学分析表明，ZD9与 XZ113的最大吸收速率（V max）相似，但 ZD9的亲和系数（K m）值较小，与 XZ149相近；在供试的4个基因型中，XZ113的光合能力较强，而 ZD9的硝酸还原酶活性较高。表明西藏一年生野生大麦的耐低氮机制可能与栽培大麦不同，为大麦低氮耐性或高效利用研究提供了新的种质材料。
이82빈서장야생대맥화16빈재배대맥위재료，이용수배시험，이생물량화담소흡수위지표，비교료타문내저담적기인형차이。결과표명저담내성적기인형차이현저，야생대맥적변이대우재배대맥。재차기출상，이감정획득적2개내저담야생대맥（XZ113화 XZ149），1개중도내저담야생대맥（XZ125）화1개내저담재배대맥품충（ZD9）위재료，연구타문재불동담농도하적생장여생리표현。담소흡수동역학분석표명，ZD9여 XZ113적최대흡수속솔（V max）상사，단 ZD9적친화계수（K m）치교소，여 XZ149상근；재공시적4개기인형중，XZ113적광합능력교강，이 ZD9적초산환원매활성교고。표명서장일년생야생대맥적내저담궤제가능여재배대맥불동，위대맥저담내성혹고효이용연구제공료신적충질재료。
Tibetan annual wild barley is an ancestor of cultivated barley and rich in genetic diversity. Moreover,some wild barley accessions show high tolerance to poor soil.In this research,82 wild barley accessions and 1 6 cultivated barley genotypes were firstly used in a hydroponic experiment,in order to compare their tolerance to low nitrogen(N).The results showed that there was a significant difference among genotypes in their low N tolerance,with the wild barley having wider variation than the cultivated barley.On the basis of the results obtained in the first experiment,two low-N tolerant wild genotypes (XZ1 13 and XZ149),one moderately tolerant wild genotype(XZ125) and one cultivated barley genotype (ZD9) were selected and used in the second experiment,to determine their growth and physiological performance under different N levels.The analysis of N-uptake dynamics showed that ZD9 and XZ1 13 had similar maximum uptake velocity (V max),and ZD9 had the smaller Michaelis-Menten constant (K m),similar to that of XZ149.Among the four barley genotypes,XZ1 13 had higher photosynthetic rate and ZD9 had higher nitrate reductase activity.The current results indicate that Tibetan wild barley has different mechanisms underlying low N tolerance from those in cultivated barley,and may provide the elite germplasm with low N tolerance or high N use efficiency for modern barley breeding.