CAJ | 학술논문

目的：初步探讨肢体缺血后处理对脑缺血/再灌注损伤的影响，验证小鼠前脑缺血再灌注损伤模型开展相关研究的可行性。方法30只小鼠随机分为3组：假手术组（A组），缺血60 min组（B组），缺血60 min ＋后处理组（C组）。用双颈总动脉阻断法建立小鼠前脑缺血/再灌注损伤模型，以可复性阻断小鼠双侧股动脉作为干预措施。实验小鼠再灌注24 h后取脑，记数海马CA1区的存活细胞数、变性细胞率。结果海马CA1区细胞存活数，A组> C组> B组（P <0.01）；细胞变性率，B组、C组都大于A组（P <0.05），但B组和 C组比较差异无统计学意义（P>0.05）。结论肢体缺血后处理可减轻脑缺血/再灌注损伤；小鼠前脑缺血再灌注损伤模型可作为开展相关研究的平台。
목적：초보탐토지체결혈후처리대뇌결혈/재관주손상적영향，험증소서전뇌결혈재관주손상모형개전상관연구적가행성。방법30지소서수궤분위3조：가수술조（A조），결혈60 min조（B조），결혈60 min ＋후처리조（C조）。용쌍경총동맥조단법건립소서전뇌결혈/재관주손상모형，이가복성조단소서쌍측고동맥작위간예조시。실험소서재관주24 h후취뇌，기수해마CA1구적존활세포수、변성세포솔。결과해마CA1구세포존활수，A조> C조> B조（P <0.01）；세포변성솔，B조、C조도대우A조（P <0.05），단B조화 C조비교차이무통계학의의（P>0.05）。결론지체결혈후처리가감경뇌결혈/재관주손상；소서전뇌결혈재관주손상모형가작위개전상관연구적평태。
Objective To investigate effects of remote ischemic postconditioning on forebrain ischemia-reperfusion injury in mice and establish a animal model for related experiments. Methods Thirty mice were randomly divided into 3 groups: sham operation group (A), ischemia for 60 min group (B), RIPC group (C). The mouse model of forebrain ischemia-reperfusion was established by blocking bilateral common carotid arteries. RIPC group received 3 cycles of 5 min reperfusion fol owed by 5 min ischemia in bilateral femoral arteries at the beginning of cerebral reperfusion. After 24h reperfusion, Comparison of the number of viable celland denatured cellrates in hippocampus gyrus CA1 area between 3 groups. Results Comparison of the number of viable cell, group A > group C> group B(P < 0.01). Compared with group A, denatured cellrates of group B and C are higher(P < 0.05). In addition, there is no significant difference between group B and C (P > 0.05). Conclusion RIPC could protect brain against forebrain ischemia-reperfusion induced injury. The mouse model of 60 min ischemia and 24h reperfusion is suitable for experiments concerning ischemia-reperfusion or RIPC.