现代仪器与医疗
現代儀器與醫療
현대의기여의료
Modern Instrument and Medical Treatment
2014年
2期
64-66
,共3页
香砂枳术丸%白术内酯Ⅰ%白术内酯Ⅲ%HPLC
香砂枳術汍%白術內酯Ⅰ%白術內酯Ⅲ%HPLC
향사지술환%백술내지Ⅰ%백술내지Ⅲ%HPLC
Xiangshazhizhu Wan%AtractylenolideⅠ%AtractylenolideⅢ%HPLC
目的：建立同时测定香砂枳术丸中白术内酯Ⅰ和Ⅲ含量方法。方法：采用DIONEX C18色谱柱（250mm×4.6mm，5μm），流动相：乙腈：甲醇：0.1%磷酸溶液（11：46：43），检测波长为222nm，柱温：35℃，流速为1.0mL·min-1。结果：白术内酯Ⅰ和Ⅲ分别在0.1～2.0μg·mL-1（γ=0.9997）和0.2～4.0μg · mL-1（γ=0.9998）浓度范围内线性关系良好，平均加样回收率分别为98.7%，98.0%；RSD 分别为0.68%、1.15%。结论：本方法简便，准确，重复性好，可用于该制剂的质量控制。
目的：建立同時測定香砂枳術汍中白術內酯Ⅰ和Ⅲ含量方法。方法：採用DIONEX C18色譜柱（250mm×4.6mm，5μm），流動相：乙腈：甲醇：0.1%燐痠溶液（11：46：43），檢測波長為222nm，柱溫：35℃，流速為1.0mL·min-1。結果：白術內酯Ⅰ和Ⅲ分彆在0.1～2.0μg·mL-1（γ=0.9997）和0.2～4.0μg · mL-1（γ=0.9998）濃度範圍內線性關繫良好，平均加樣迴收率分彆為98.7%，98.0%；RSD 分彆為0.68%、1.15%。結論：本方法簡便，準確，重複性好，可用于該製劑的質量控製。
목적：건립동시측정향사지술환중백술내지Ⅰ화Ⅲ함량방법。방법：채용DIONEX C18색보주（250mm×4.6mm，5μm），류동상：을정：갑순：0.1%린산용액（11：46：43），검측파장위222nm，주온：35℃，류속위1.0mL·min-1。결과：백술내지Ⅰ화Ⅲ분별재0.1～2.0μg·mL-1（γ=0.9997）화0.2～4.0μg · mL-1（γ=0.9998）농도범위내선성관계량호，평균가양회수솔분별위98.7%，98.0%；RSD 분별위0.68%、1.15%。결론：본방법간편，준학，중복성호，가용우해제제적질량공제。
Objective:To establish a method for the determination of atractylenolideⅠand atractylenolide Ⅲ in Xiangshazhizhu Wan. Method:The reversed phase HPLC system consisting the DIONEX C18(250mm×4.6mm,5μm)column,the mobile phase consisted of Acetonitrile (11) -Methanol(46)- 0.1%H3PO4(43), the UV detector was set at 222nm, the flow rate was 1.0mL·min-1,the column temperature was 35℃. Results The linear response range were0.1~2.0μg·mL-1(γ=0.9997)and 0.2~4.0μg·mL-1(γ=0.9998),respectively. The average recovery of atractylenolideⅠand atractylenolideⅢwere 98.7%and 98.0%, RSD were 0.68%,1.15%respectively. Conclusions:The assay demonstrated that the method had adequate and selectively to measure the concentrations of simple, accurate and repeatable, it could be used as a reliable tool for the determination of atractylenolideⅠand atractylenolideⅢin Xiangshazhizhu Wan.
