CAJ | 학술논문

目的：采用纳米均相时间分辨荧光免疫技术(amplified luminescent proximity homogeneous assay, Alpha-LISA)建立高灵敏检测盐酸克伦特罗(clenbuterol, CLB)残留的方法。方法在发光微粒上固定 CLB,与游离的CLB共同竞争固定在感光微粒上的CLB单克隆抗体,优化检测条件并进行方法学考核。结果该方法的灵敏度为0.04 ng/mL。猪肉样品回收率在83%~109%之间,牛肉样品回收率在92%~112%之间；猪肉样品检测的批内变异系数CV<10%、批间变异系数CV<15%。与特布他林、沙丁胺醇的交叉反应率分别为34.5%、25.7%,与莱克多巴胺、妥布特罗、齐帕特罗无交叉反应。结论 CLB-AlphaLISA 检测肉类中克伦特罗残留具有简单、快速、灵敏性高、特异性强、稳定等特点,具有广阔的应用前景。
목적：채용납미균상시간분변형광면역기술(amplified luminescent proximity homogeneous assay, Alpha-LISA)건립고령민검측염산극륜특라(clenbuterol, CLB)잔류적방법。방법재발광미립상고정 CLB,여유리적CLB공동경쟁고정재감광미립상적CLB단극륭항체,우화검측조건병진행방법학고핵。결과해방법적령민도위0.04 ng/mL。저육양품회수솔재83%~109%지간,우육양품회수솔재92%~112%지간；저육양품검측적비내변이계수CV<10%、비간변이계수CV<15%。여특포타림、사정알순적교차반응솔분별위34.5%、25.7%,여래극다파알、타포특라、제파특라무교차반응。결론 CLB-AlphaLISA 검측육류중극륜특라잔류구유간단、쾌속、령민성고、특이성강、은정등특점,구유엄활적응용전경。
Objective A direct competitive amplified luminescent proximity homogeneous assay (Alpha-LISA) for detecting clenbuterol(CLB) was established. Methods CLB was coated on emitting particles, and competed with sample CLB to anti-CLB which was coated on photosensitive particles. The analytical perfor-mance and optinal test conditions of the method were studied. Results The sensitivity of the assay was 0.04 ng/mL. The recoveries of the determination for CLB in pork and beer were respectively 83%~109% and 92%~112%. The CV of intra-and inter-assay were <10% and <15% respectively. The cross-reactivity of the CLB-AlphaLISA with ractopamine, tulobuterol and zilpaterol was negligible, while that with terbutaline and salbutamol was 34.5% and 25.7% respectively. Conclusion The CLB-AlphaLISA had the characteristics of excellent specificity and sensitivity, and good precision and economy. It could be widely used in rapid screen-ing for CLB contamination in meat or foods in the future.