CAJ | 학술논문

对蛹虫草纤溶酶的酶学性质进行了研究，结果表明，其纤溶活性随温度（≤45℃）的升高和保温时间的延长，逐渐下降，当温度超过50℃时，则随温度的升高和保温时间的延长急剧下降；蛹虫草纤溶酶的最适pH为7.0，在碱性条件下纤溶活性相对稳定；金属离子Ca2+和Fe2+对蛹虫草纤溶酶的活性有显著激活作用；抑制剂中PMSF对蛹虫草纤溶酶活性的抑制作用较小，而Aprotinin、EDAT和 EGTA对其纤溶活性均有较显著的抑制作用，说明蛹虫草纤溶酶可能不同于之前报道的纳豆激酶（丝氨酸蛋白酶），而是一种全新的蛋白酶，但对这一点还需要进一步的研究验证.
대용충초섬용매적매학성질진행료연구，결과표명，기섬용활성수온도（≤45℃）적승고화보온시간적연장，축점하강，당온도초과50℃시，칙수온도적승고화보온시간적연장급극하강；용충초섬용매적최괄pH위7.0，재감성조건하섬용활성상대은정；금속리자Ca2+화Fe2+대용충초섬용매적활성유현저격활작용；억제제중PMSF대용충초섬용매활성적억제작용교소，이Aprotinin、EDAT화 EGTA대기섬용활성균유교현저적억제작용，설명용충초섬용매가능불동우지전보도적납두격매（사안산단백매），이시일충전신적단백매，단대저일점환수요진일보적연구험증.
The characterization of fibrinolytic enzyme of Cordyceps militaris was characterized in this article. Results show that,the fibrinolytic activity of C. militaris decreased slowly with the increase of temperature(≤45 ℃) and extension of holding time. But the fibrinolytic activity decreased sharply when the temperature reached higher than 50 ℃. The optimal reaction pH value is 7.0. The fibrinolytic activity has better stability in alkaline environment than in acid environment. The fibrinolytic activity was strongly activated by the presence of Ca2+and Fe2+. In addition, the fibrinolytic activity was inhibited slightly by PMSF. The Aprotinin,EDAT and EGTA all have significant inhibi-tions on fibrinolytic activity. It was deduced that the fibrinolytic enzyme of C. militaris is different with the nattokinase (a serine protease) reported earlier,but a new protease. Further research is needed to confirm the results above.