CAJ | 학술논문

目的：探讨红景天对大鼠脑缺血再灌注后脑组织中c-Fos表达水平及神经细胞凋亡的影响。方法 Wistar大鼠120只，分为假手术组、模型组和干预组(红景天)，各组再分为3 h、6 h、12 h、24 h、48 h共5个亚组，每个亚组8只大鼠。后两组采用大脑中动脉线栓法制备局灶性脑缺血再灌注模型，干预组术前红景天每天0.672 g/kg灌胃15 d。参考Longa法在大鼠麻醉清醒后进行评分，应用免疫组化、TUNEL法检测各组c-Fos表达及神经细胞凋亡。结果模型组和干预组缺血脑组织中c-Fos表达明显增加(P<0.01)，于再灌注48 h达高峰；与模型组相比，干预组c-Fos表达减少(P<0.05)，神经元凋亡明显减少(P<0.01)，神经功能缺损评分下降(P<0.05)。结论红景天能抑制大鼠脑缺血再灌注后脑组织中c-Fos表达，减少神经元凋亡，减轻缺血再灌注损伤。
목적：탐토홍경천대대서뇌결혈재관주후뇌조직중c-Fos표체수평급신경세포조망적영향。방법 Wistar대서120지，분위가수술조、모형조화간예조(홍경천)，각조재분위3 h、6 h、12 h、24 h、48 h공5개아조，매개아조8지대서。후량조채용대뇌중동맥선전법제비국조성뇌결혈재관주모형，간예조술전홍경천매천0.672 g/kg관위15 d。삼고Longa법재대서마취청성후진행평분，응용면역조화、TUNEL법검측각조c-Fos표체급신경세포조망。결과모형조화간예조결혈뇌조직중c-Fos표체명현증가(P<0.01)，우재관주48 h체고봉；여모형조상비，간예조c-Fos표체감소(P<0.05)，신경원조망명현감소(P<0.01)，신경공능결손평분하강(P<0.05)。결론홍경천능억제대서뇌결혈재관주후뇌조직중c-Fos표체，감소신경원조망，감경결혈재관주손상。
Objective To investigate the effect of Rhodiola Rosae P. E on the expression of c-Fos and the apoptosis of neuronal in rat brain after ischemia/reperfusion (I/R) injury. Methods 120 Wistar rats were divided into sham group, model group and intervention group, subgrouped as 3 h, 6 h, 12 h, 24 h, and 48 h. The model and intervention groups underwent middle cerebral artery occlusion, and the latter accepted Rhodiola 0.672 g/kg daily for 15 d. They were assessed with Longa's score. The expression of c-Fos and apoptosis of neuronal were measured with immunohistochemistry and TUNEL. Results The expression of c-Fos in the ischemic area increased after cerebral isch-emia/reperfusion (P<0.01), peaking at 24 h after injury, and decreased in the intervention group (P<0.01), with the decrease of neuron apop-tosis and Longa's score (P<0.01). Conclusion Rhodiola protects the brain tissue against ischemia/reperfusion injury by inhibiting the expres-sion of c-Fos and apoptosis in rats.