CAJ | 학술논문

目的：观察电针对兔腰肌急性钝挫伤后碱性成纤维细胞生长因子(bFGF)、结蛋白(Desmin)以及细胞外信号调节激酶(ERK1/2)表达的影响，探讨电针对兔腰肌损伤后组织再生修复影响的可能机制。方法40只雄性新西兰大耳兔随机分为空白组、模型组、电针委中组、电针局部组，每组10只。采用钝挫伤方法造成兔腰肌损伤模型并对各组动物进行外表观察评分。造模后电针治疗2周，HE染色观察腰肌组织形态改变，免疫组化染色观察腰肌bFGF的表达，Western blotting法检测腰肌Desmin与ERK1/2蛋白表达。结果模型组、电针委中组与电针局部组外表观察评分均明显高于空白组(P<0.01)。组织形态学评分由高到低依次为模型组、电针局部组、电针委中组、空白组(P<0.05)，腰肌bFGF的表达依次为电针局部组、电针委中组、模型组、空白组，Desmin的表达为电针局部组=电针委中组、空白组=模型组(P<0.05)，ERK1/2的表达为电针局部组=电针委中组、模型组、空白组(P<0.05)。结论电针可能通过上调bFGF/ERK信号通路的表达，促进兔腰肌急性钝挫伤后的组织修复。
목적：관찰전침대토요기급성둔좌상후감성성섬유세포생장인자(bFGF)、결단백(Desmin)이급세포외신호조절격매(ERK1/2)표체적영향，탐토전침대토요기손상후조직재생수복영향적가능궤제。방법40지웅성신서란대이토수궤분위공백조、모형조、전침위중조、전침국부조，매조10지。채용둔좌상방법조성토요기손상모형병대각조동물진행외표관찰평분。조모후전침치료2주，HE염색관찰요기조직형태개변，면역조화염색관찰요기bFGF적표체，Western blotting법검측요기Desmin여ERK1/2단백표체。결과모형조、전침위중조여전침국부조외표관찰평분균명현고우공백조(P<0.01)。조직형태학평분유고도저의차위모형조、전침국부조、전침위중조、공백조(P<0.05)，요기bFGF적표체의차위전침국부조、전침위중조、모형조、공백조，Desmin적표체위전침국부조=전침위중조、공백조=모형조(P<0.05)，ERK1/2적표체위전침국부조=전침위중조、모형조、공백조(P<0.05)。결론전침가능통과상조bFGF/ERK신호통로적표체，촉진토요기급성둔좌상후적조직수복。
Objective To observe the effect of electroacupuncture on basic fibroblast growth factor (bFGF), Desmin and extracellular signal-regulated kinase (ERK1/2) expression of rabbits with acute lumbar muscle contusion, and discuss the possible mechanism of muscle tissue regeneration and repair. Methods 40 male New Zealand rabbits were randomly divided into blank group (BG, n=10), model group (MG, n=10), Weizhong (BL40) electroacupuncture group (WG, n=10) and local electroacupuncture group (LG, n=10). Lumbar muscle inju-ry was established with blunt trauma, and the rabbits were assessed with Appearance Score. The WG and LG accepted electroacupuncture for 2 weeks after modeling. The lumbar muscle of each animal was stained with HE to observe pathological changes, and immunohistochem-ical staining was used to observe bFGF expression, and Western blotting was used to detect the expression of Desmin and ERK1/2 protein. Results Appearance Score was more in the MG, WG and LG than in the BG (P<0.01). Histological score ranked from more to less as MG, LG, WG and BG (P<0.05). The expression of bFGF ranked as LG, WG, MG and BG (P<0.01), Desmin ranked as WG=LG and BG=MG (P<0.05), ERK1/2 as LG=WG, MG and BG (P<0.05). Conclusion Electroacupuncture could promote the regeneration and repair of tissue after acute lumbar muscle contusion, which may be related to the up-regulation of bFGF/ERK signaling pathway.