CAJ | 학술논문

目的：观测电针大鼠双侧合谷穴(LI04)对局灶性脑缺血再灌注后脑内CD34表达及血管新生的影响。方法90只大鼠随机分为正常组(n=6)、模型组(n=42)和电针组(n=42)。模型组和电针组采用线栓法制备大鼠局灶性脑缺血再灌注模型，电针组采用电针治疗。局灶性脑缺血1h后再灌注，观察再灌注后2h、12h、24h、3d、7d、14d、21d共7个时相点大鼠神经症状学评分，采用HE染色观察并计算梗死体积和毛细血管密度，免疫组织化学法检测CD34的表达。结果电针组与模型组比较，再灌注后各时间点神经症状学评分降低(P<0.05)。电针组梗死体积显著小于模型组(P<0.001)。与模型组相比，电针组毛细血管密度再灌注后2 h开始增加(P<0.05)，再灌注后24 h到再灌注后14 d均较模型组明显增加(P<0.01)。电针组再灌注后3 d CD34阳性细胞表达较模型组明显增加(P<0.01)，7 d增加达高峰(P<0.01)，14 d仍有显著性差异(P<0.05)。结论电针能促进局灶性脑缺血再灌注后CD34的表达，增加毛细血管密度，促进血管新生。
목적：관측전침대서쌍측합곡혈(LI04)대국조성뇌결혈재관주후뇌내CD34표체급혈관신생적영향。방법90지대서수궤분위정상조(n=6)、모형조(n=42)화전침조(n=42)。모형조화전침조채용선전법제비대서국조성뇌결혈재관주모형，전침조채용전침치료。국조성뇌결혈1h후재관주，관찰재관주후2h、12h、24h、3d、7d、14d、21d공7개시상점대서신경증상학평분，채용HE염색관찰병계산경사체적화모세혈관밀도，면역조직화학법검측CD34적표체。결과전침조여모형조비교，재관주후각시간점신경증상학평분강저(P<0.05)。전침조경사체적현저소우모형조(P<0.001)。여모형조상비，전침조모세혈관밀도재관주후2 h개시증가(P<0.05)，재관주후24 h도재관주후14 d균교모형조명현증가(P<0.01)。전침조재관주후3 d CD34양성세포표체교모형조명현증가(P<0.01)，7 d증가체고봉(P<0.01)，14 d잉유현저성차이(P<0.05)。결론전침능촉진국조성뇌결혈재관주후CD34적표체，증가모세혈관밀도，촉진혈관신생。
Objective To observe the effect of electroacupuncture at Hegu (LI04) on capillary density and expression of CD34 after focal cerebral ischemia/reperfusion in rats. Methods 90 rats were randomly divided into normal group (n=6), model group (n=42) and electroacu-puncture group (n=42). A rat model of focal cerebral ischemia/reperfusion was made by filament occlusion in the model group and the elec-troacupuncture group. And the electroacupuncture group received electroacupuncture. They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after reperfusion 1 h followed ischemia. HE staining was used to observe and calculate the infarct volume and capillary density, and immu-nohistology was used to detect the expression of CD34 after neurologic symptoms rating. Results Compared with the model group, the neuro-logic symptoms score significantly decreased 2 h after reperfusion in the electroacupuncture group (P<0.01), and still decreased 21 d after re-perfusion (P<0.05). The infarct volume significantly was smaller in the electroacupuncture group than in the model group (P<0.01). The cap-illary density was higher 2 h, 24 h, 3 d, 7 d, 14 d after reperfusion in the electroacupuncture group than in the model group (P<0.05). Com-pared with the model group, the expression of CD34 significantly increased 3 d after reperfusion in the electroacupuncture group (P<0.01), and it reached a peak at 7 d after reperfusion (P<0.01), and still higher at 14 d. Conclusion Electroacupuncture can promote the expression of CD34 and the capillary density, and stimulate the angiogenesis after focal ischemia/reperfusion.