CAJ | 학술논문

采用动物组织块培养法对四鳃鲈（Trachidermus fasciatus）内脏组织进行离体培养研究.对培养条件进行了优化，比较了不同培养基（DMEM、M199）、不同生长条件（加生长因子、不加生长因子）和不同培养条件（CO2培养箱、恒温培养箱）下离体培养细胞的生长情况.结果显示：内脏组织接种后3~5 h，细胞迁移伸展，6 d后生长细胞集群汇合，来源于不同组织的再生细胞呈现不同的形态.在M199培养基中添加青链霉素抗生素（0.28%）及10%小牛血清和1%碱性成纤维生长因子、控温27℃、pH值为7.2~7.4并在CO2（5%）培养箱中培养的内脏细胞生长最快.不同来源的组织培养中肝细胞在离体培养中细胞增殖生长速度最快.
채용동물조직괴배양법대사새로（Trachidermus fasciatus）내장조직진행리체배양연구.대배양조건진행료우화，비교료불동배양기（DMEM、M199）、불동생장조건（가생장인자、불가생장인자）화불동배양조건（CO2배양상、항온배양상）하리체배양세포적생장정황.결과현시：내장조직접충후3~5 h，세포천이신전，6 d후생장세포집군회합，래원우불동조직적재생세포정현불동적형태.재M199배양기중첨가청련매소항생소（0.28%）급10%소우혈청화1%감성성섬유생장인자、공온27℃、pH치위7.2~7.4병재CO2（5%）배양상중배양적내장세포생장최쾌.불동래원적조직배양중간세포재리체배양중세포증식생장속도최쾌.
The primary visceral cells was initiated from visceral tissues explants of Trachidermus fasciatus by us-ing tissue block culture method. The explants were cultured in different medium (DMEM or M199) with different growing conditions (with/without growth factor) and different culture conditions (CO2 inculzertor or Constant tem-perature inculzertor), aiming at comparing the influence of culture methods and conditions on the growth of cells and figuring out the optimal. The results showed that the cells migrated out around the tissues after 3~5 hours and the cells gathered together after 6 days. The regeneration cells from virous tissues presented different cellu-lar morphology. The visceral cells grew better in the medium of M199 supplemented with moderate amount of an-tibiotics, 10% new born calf serum,1% basic fibroblast growth factor and incubated at temperature 27 ℃, pH 7.2~7.4 and 5% CO2 than in other conditions. The hepatocytes grew faster in vitro than any other cells from dif-ferent sources.