CAJ | 학술논문

目的：探讨舌癌Tca8113细胞能否分泌exosomes，为舌癌的免疫治疗寻找新方法。方法采用超速离心及差异密度梯度离心法，从Tca8113细胞中分离并纯化exosomes，通过透射电子显微镜观察其超微结构，蛋白质印迹法分析其表面蛋白成分。结果从Tca8113细胞中成功分离并纯化出exosomes，其结构及携带蛋白与其他细胞来源的相似，并发现其分泌的exosomes还携带有肿瘤抗原。结论舌癌Tca8113细胞能分泌exosomes，而且其携带多种与肿瘤免疫相关的蛋白可能具有抗肿瘤作用。
목적：탐토설암Tca8113세포능부분비exosomes，위설암적면역치료심조신방법。방법채용초속리심급차이밀도제도리심법，종Tca8113세포중분리병순화exosomes，통과투사전자현미경관찰기초미결구，단백질인적법분석기표면단백성분。결과종Tca8113세포중성공분리병순화출exosomes，기결구급휴대단백여기타세포래원적상사，병발현기분비적exosomes환휴대유종류항원。결론설암Tca8113세포능분비exosomes，이차기휴대다충여종류면역상관적단백가능구유항종류작용。
Objective This study aims to verify whether Tca8113 can secrete exosomes, and to identify a new method for oral tumor immunotherapy. Methods Exosomes were isolated and purified from the culture supernatant of Tca8113 by ultraspeed centrifugation and density gradient centrifugation, and were subsequently confirmed using transmission electron microscopy and Western blot. Results Exosomes were successfully isolated and purified from the culture supernatant of Tca8113. Tca8113 contained protein and had a structure similar to other cells, as reported in previous studies. Conclusion Tca8113 can secrete exosomes, and contains numerous types of proteins that are associated with tumor immunity.